COQ3 (NM_017421) Human Mass Spec Standard

CAT#: PH309576

COQ3 MS Standard C13 and N15-labeled recombinant protein (NP_059117)

Product Images

Coomassie blue staining of purified COQ3 protein (Cat# TP309576). The protein was produced from HEK293T cells transfected with COQ3 cDNA clone (Cat# RC209576) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Tag: C-Myc/DDK
• Species: Human
• Expression Host: HEK293
• Expression cDNA Clone or AA Sequence: RC209576
• Predicted MW: 41 kDa
• Protein Sequence:
  Sequence Link

  >RC209576 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MWSGRKLGSSGGWFLRVLGPGGCNTKAARPLISSAVYVKNQLSGTLQIKPGVFNEYRTIWFKSYRTIFSC
  LNRIKSFRYPWARLYSTSQTTVDSGEVKTFLALAHKWWDEQGVYAPLHSMNDLRVPFIRDNLLKTIPNHQ
  PGKPLLGMKILDVGCGGGLLTEPLGRLGASVIGIDPVDENIKTAQCHKSFDPVLDKRIEYRVCSLEEIVE
  ETAETFDAVVASEVVEHVIDLETFLQCCCQVLKPGGSLFITTINKTQLSYALGIVFSEQIAGIVPKGTHT
  WEKFVSPETLESILESNGLSVQTVVGMLYNPFSGYWHWSENTSLNYAAHAVKSRVQEHPASAEFVLKGET
  EELQANACTNPAVHEKLKK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: 50 ug/ml as determined by BCA
• Labeling Method: Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
• Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3. Store at -80°C. Avoid repeated freeze-thaw cycles. Stable for 3 months from receipt of products under proper storage and handling conditions.

Reference Data

• RefSeq: NP_059117
• RefSeq Size: 1265
• RefSeq ORF: 1107
• Synonyms: bA9819.1; DHHBMT; DHHBMTASE; UG0215E05
• Locus ID: 51805
• UniProt ID: Q9NZJ6
• Cytogenetics: 6q16.2
• Summary: Ubiquinone, also known as coenzyme Q, or Q, is a critical component of the electron transport pathways of both eukaryotes and prokaryotes (Jonassen and Clarke, 2000 [PubMed 10777520]). This lipid consists of a hydrophobic isoprenoid tail and a quinone head group. The tail varies in length depending on the organism, but its purpose is to anchor coenzyme Q to the membrane. The quinone head group is responsible for the activity of coenzyme Q in the respiratory chain. The S. cerevisiae COQ3 gene encodes an O-methyltransferase required for 2 steps in the biosynthetic pathway of coenzyme Q. This enzyme methylates an early coenzyme Q intermediate, 3,4-dihydroxy-5-polyprenylbenzoic acid, as well as the final intermediate in the pathway, converting demethyl-ubiquinone to coenzyme Q. The COQ3 gene product is also capable of methylating the distinct prokaryotic early intermediate 2-hydroxy-6-polyprenyl phenol. [supplied by OMIM, Mar 2008]
• Protein Families: Druggable Genome
• Protein Pathways: Metabolic pathways, Ubiquinone and other terpenoid-quinone biosynthesis