VEGF-E Orf Virus Protein

CAT#: DA3516

VEGF-E orf virus recombinant protein, 5 µg

Specifications

Product Data

• Species: Orf Virus
• Expression Host: E. coli
• Predicted MW: 35 kDa
• Purity: >90% pure by SDS-PAGE and visualised by silver stain.
• Buffer: Presentation State: Purified
  State: Lyophilized
  Buffer System: PBS, pH 7.4 without stabilizers.
• Bioactivity: Biological: The ED50 for stimulation of 3H-thymidine incorporation and cell proliferation by human umbilical vein endothelial cells for VEGF-E has been determined to be in the range of 5-20 ng/ml.
  Specific: 2 x 10e5 units/mg
• Endotoxin: < 0.1 ng per µg of VEGF-E
• Reconstitution: Restore in PBS or medium containing at least 0.1% human or bovine serum albumin to a concentration not lower than 50 µg/ml.
  (VEGF-E is soluble in water and most aqueous buffers).
• Preparation: Lyophilized
• Protein Description: Recombinant ov-VEGF-E (Orf virus). A DNA sequence encoding the mature variant of ovVEGF-E isolate D1701 (Dehio et al., 1999; GenBank accession No. AF106020) was expressed in E. coli as a 132 amino acid residue fusion protein with an N-terminal His-tag sequence and a thrombin cleavage site. Recombinant VEGF-E homodimer was dimerized in vitro and has a predicted mass of approximately 35 kDa.
• Note: Centrifuge vial before opening!
• Storage: Store lyophilized VEGF-E at -20°C to -70°C.
  Reconstituted VEGF-E should be stored in working aliquots at -20°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: six months from despatch.