Dbt (NM_010022) Mouse Recombinant Protein

CAT#: TP507721

Purified recombinant protein of Mouse dihydrolipoamide branched chain transacylase E2 (Dbt), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug

Specifications

Product Data

• Species: Mouse
• Expression Host: HEK293T
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  A DNA sequence from Mouse cDNA ORF Clone, MR207721, encoding Mouse full-length Dbt.
• Tag: C-MYC/DDK
• Predicted MW: 53.3 kDa
• Concentration: >50 ug/mL as determined by microplate BCA method
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Storage: Store at -80°C after receiving vials.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.

Reference Data

• RefSeq: NP_034152
• Locus ID: 13171
• UniProt ID: P53395
• Cytogenetics: 3 50.37 cM
• Refseq Size: 3292
• Refseq ORF: 1449
• Synonyms: BCKAD-E2; D3Wsu60e
• Summary: The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of three enzymatic components: branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltransferase (E2) and lipoamide dehydrogenase (E3). Within this complex, the catalytic function of this enzyme is to accept, and to transfer to coenzyme A, acyl groups that are generated by the branched-chain alpha-keto acid decarboxylase component. [UniProtKB/Swiss-Prot Function]