CD82 Human shRNA Plasmid Kit (Locus ID 3732)

CAT#: TL314064

CD82 - Human, 4 unique 29mer shRNA constructs in lentiviral GFP vector

Product Images

GFP signal was observed under microscope at 48 hours after transduction of TL314064A virus into HEK293 cells. TL314064A virus was prepared using lenti-shRNA TL314064A and TR30037 packaging kit.

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  GFP signal was observed under microscope at 48 hours after transduction of TL314064B virus into HEK293 cells. TL314064B virus was prepared using lenti-shRNA TL314064B and TR30037 packaging kit.

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  GFP signal was observed under microscope at 48 hours after transduction of TL314064C virus into HEK293 cells. TL314064C virus was prepared using lenti-shRNA TL314064C and TR30037 packaging kit.

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  GFP signal was observed under microscope at 48 hours after transduction of TL314064D virus into HEK293 cells. TL314064D virus was prepared using lenti-shRNA TL314064D and TR30037 packaging kit.

Specifications

Product Data

• Locus ID: 3732
• Synonyms: 4F9; C33; GR15; IA4; KAI1; R2; SAR2; ST6; TSPAN27
• Vector: pGFP-C-shLenti
• E. coli Selection: Chloramphenicol
• Mammalian Cell Selection: Puromycin
• Format: Lentiviral plasmids
• Kit Components: CD82 - Human, 4 unique 29mer shRNA constructs in lentiviral GFP vector(Gene ID = 3732). 5µg purified plasmid DNA per construct
  Non-effective 29-mer scrambled shRNA cassette in pGFP-C-shLenti Vector, TR30021, included for free.
• RefSeq: NM_001024844, NM_002231, BC000726, BC001821
• Summary: 'This metastasis suppressor gene product is a membrane glycoprotein that is a member of the transmembrane 4 superfamily. Expression of this gene has been shown to be downregulated in tumor progression of human cancers and can be activated by p53 through a consensus binding sequence in the promoter. Its expression and that of p53 are strongly correlated, and the loss of expression of these two proteins is associated with poor survival for prostate cancer patients. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]'
• shRNA Design: These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, align it with our published shRNA design sequences. If these do not align, please utilize our custom shRNA service
• Performance Guaranteed: OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples.
  
  For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred).