pCas-Guide-Nickase (D10A)

CAT#: GE100019

pCas-Guide-Nickase vector for gRNA cloning (with Cas9 D10A expression) (10 µg)

Reconstitution Protocol

USD 490.00

In Stock*

Size
    • 10 ug

Product Images

Specifications

Product Data
Function Cas9 Nickase
Features

All-in-one vector

  • Expresses human codon-optimized Cas9 nickase D10A mutant, C-terminal Myc-DDK tagged
  • BamH I and BsmB I can be used for genomic target sequence cloning
  • Expresses gRNA containing the inserted target sequence and the gRNA scaffold
  • CF3 primer can be used to sequence the target sequence after cloning
  • Download vector sequence

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