Anti-Bordetella pertussis IgA ELISA Kit

CAT#: EA101051

Anti-Bordetella pertussis IgA ELISA Kit

Specifications

Product Data

• Format: 96-well strip plate
• Assay Type: Solid Phase Sandwich ELISA
• Assay Length: 3 hours
• Signal: Colorimetric
• Sample Type: Cell culture supernatant, serum, plasma
• Sample Volume: 10 ul
• Specificity: Natural and recombinant human Anti-Bordetella pertussis IgA
• Reactivities: Human
• Interference: No significant interference observed with available related molecules.
• Components: See the product information for more details
• Background: The Bordetella pertussis / toxin IgA ELISA Kit is a solid phase enzyme-linked immunosorbent assay (ELISA). Microtiter wells as a solid phase are coated with Bordetella pertussis and Bordetella pertussis toxin antigen. Diluted sample specimens and ready-for-use controls are pipetted into these wells. During incubation Bordetella pertussis and Bordetella pertussis toxin-specific antibodies in specimens and controls are bound to the immobilized antigens. After a washing step to remove unbound sample and control material horseradish peroxidase conjugated anti-human IgA antibodies are dispensed into the wells. During a second incubation this anti-IgA conjugate binds specifically to IgA antibodies resulting in the formation of enzyme-linked immune complexes. After a second washing step to remove unbound conjugate the immune complexes formed (in case of analyte presence) are detected by incubation with TMB substrate and development of a blue color. The blue color turns into yellow by stopping the enzymatic indicator reaction with sulfuric acid. The intensity of this color is directly proportional to the amount of Bordetella pertussis and Bordetella pertussis toxin-specific IgA antibody in the sample specimen. Absorbance at 450 nm is read using an ELISA microtiter plate reader.