Colorectal Cancer

Colorectal Cancer (CRC), more frequently detected in western countries, is caused by mutations in target oncogenes, tumor suppressor genes and genes related to DNA repair mechanisms. CRC is classified into 3 types depending on the origin of the mutation: Sporadic (70%), inherited (5%) and familial (25%)^[1].

Colorectal cancer antibody sampler panel- customizable

• PMS2: TA506658S

  30 ul

  PMS2 mouse monoclonal antibody, clone OTI2G5 (formerly 2G5)

• MSH6: UM870148

  30 ul

  MSH6 mouse monoclonal antibody,clone UMAB258

• MSH2: UM870149

  30 ul

  MSH2 mouse monoclonal antibody,clone UMAB259

• MLH1: UM870083

  30 ul

  MLH1 mouse monoclonal antibody,clone UMAB191

Sporadic cancer: These cancers are derived from point mutations and account for 70% of CRC. The occurrence of mutations follow a specific sequence which is followed by a morphological sequence.

Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-MSH2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120?C for 2.5 min,?UM800149) (1:2400)

Immunohistochemical staining of paraffin-embedded human colon adenocarcinoma tissue using anti-MSH6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH9.0) at 120?C for 3min,?UM800148) (1:50)

Immunohistochemical staining of paraffin-embedded Human Kidney tissue using anti-MLH1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 110?C for 10min,?UM800083) (1:200)

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-PMS2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120?C for 3min, TA506658)

Adenomatous polyposis coli (APC), a tumor suppressor gene, is the first gene to be mutated leading to the formation of a non-malignant adenomas called polyps. 15% of all adenomas progress to full blown carcinomas over 10 years.

Key genes mutated in Sporadic CRC

APC KRAS TP53 DCC SMAD4 BRAF

Mutant ORF clones	Ultra-Specific Antibodies & other monoclonals	OEL Controls (controls for western blot)	CytoSections (controls for IHC, ICC)
	APC - Antibodies	APC ? Overexpression lysates	APC - CytoSections
KRAS Mutants (G12C, G12V, G13D & more)	KRAS - Antibodies	KRAS ? Overexpression lysates	KRAS - CytoSections
TP53 mutants	TP53 Ultra-specific antibodies	TP53 OEL controls	TP53- CytoSections
	DCC - Antibodies	DCC ? Overexpression lysates	DCC - CytoSections

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Inherited cancer: In the inherited form of CRC, one of the mutation is inherited. The 2nd mutation triggers the apparition of the tumor cell and finally resulting in carcinoma. Inherited CRC is classified into two groups: polyposis and non-polyposis forms. Polyposis mainly involved familial adenomatous polyposis (FAP) which is characterized by the formation of multiple polyps in the colon. Hereditary non-polyposis colorectal cancer (HNPCC) is related to mutation in DNA repair mechanisms. Lynch syndrome is the main cause of hereditary non-polyposis colorectal cancer.

Key genes mutation in Lynch syndrome

MLH1 MSH2 MSH6 PMS1

Mutant ORF clones	Monoclonal Antibodies (Mouse , Rabbit)	OEL Controls (controls for western blot)	CytoSections (controls for IHC, ICC)
MLH1- Mutants	MLH1 Antibodies	MLH1 OEL controls	MLH1 CytoSections
MSH2 Mutants	MSH2 Antibodies	MSH2 OEL controls	MSH2-CytoSections
MSH6 Mutants	MSH6 Antibodies		MSH6 CytoSections
	PMS1 Antibodies	PMS1 OEL Controls	PMS1 CytoSections

Tissue samples for Colorectal Cancer

Frozen Sections Tissue Protein Lysates Total RNA FFPE Sections Tissue Genomic DNA

References:

1. M?rmol I, S?nchez-de-Diego C, Pradilla Dieste A, Cerrada E, Rodriguez Yoldi MJ. Colorectal Carcinoma: A General Overview and Future Perspectives in Colorectal Cancer. Int J Mol Sci. 2017 Jan 19;18(1):197.