SARS-CoV-2 (2019-nCoV) Research Tools

COVID-19 is caused by the coronavirus SARS-CoV-2, also called 2019-nCoV. The viral spike (S) protein - host cell ACE2 receptor interaction is key to a successful infection of the host cell.

Image 1: Coronavirus with Spike Image
Coronavirus with Spike
Image 1: Coronavirus with Spike Image

Spike Protein (S): SARS-CoV2 enters the cells through the Spike mediated interaction with the ECD domain of the ACE2 cell receptor. A recombinant fusion protein (RBD of Spike protein and ECD of membrane protein) can a great tool to investigate this interaction. Learn more about the purified recombinant Spike Protein (S)

Recently several SARS-CoV-2 variants with mutation in the spike (S)-protein has been identified. Of all the variants analyzed, variants carrying D614G mutation was significantly more infectious than others. Some of the variants with amino acid change in receptor binding domain (RBD) were less infectious and some were resistant to neutralizing antibodies. Understanding the impact of these mutations is of great value for development of vaccines and therapeutic antibodies.

Key concerned variants: Y453F N439K N501Y D614G
Full list of SARS-CoV-2 Spike Protein Variants

SARS-CoV-2 RBD Neutralizing Monoclonal Antibody:SARS-Cov-2 RBD neutralizing antibody (Cat# TA814512) inhibits human ACE2 protein (Cat# TP701115) binding to SARS-CoV-2 RBD protein TP701119). The IC50 is typically 0.91 nM.

Find out more about S Protein RBD and S-Protein’ research tools. Try out our image-click


Image 4: ACE-2 Receptor Protein Image
ACE-2 Receptor Protein

ACE-2 receptor: ACE-2 is the host cell receptor responsible for mediating infection by SARS-CoV-2.

Tools for analyzing ACE-2 protein

  1. Human Recombinant Protein Angiotensin Converting Enzyme (ACE2)
  2. ACE-2 specific Mouse Monoclonal Antibody
  3. ACE-2 Human ORF clones

Find out more about the additional ACE-2 specific tools here


Image 5: Furin Protein Image
ACE-2 Receptor Protein

Furin: It is a protease present in many human organs that recognizes and activates a specific site on the SARS-CoV-2 Spike protein, thus facilitating a tighter binding to the ACE-2 receptor and might play a role in the higher infection rate [3].

How can you analyze Furin? Learn about the tools for analyzing Furin.


Image 6: IL2 Pathway Image
IL2 Pathway

Cytokines: Studies have shown a strong correlation between severity of the disease and concentrations of IL2, IL7, IL10, GCSF, MCP1 and TNF alpha [1].

Tools to analyze the 6 cytokines associated Cytokine Storm:
IL2 | IL7 | IL10 | GCSF | MCP1 | TNF alpha

References:

  1. https://link.springer.com/article/10.1007/s12098-020-03263-6
  2. https://science.sciencemag.org/content/367/6483/1260
  3. https://science.sciencemag.org/content/367/6483/1260