Tools to Investigate SARS-CoV-2 Infection
COVID-19 is caused by the coronavirus SARS-CoV-2, also called 2019-nCoV. The viral spike (S) protein - host cell ACE2 receptor interaction is key to a successful infection of the host cell.
Spike Protein (S): SARS-CoV2 enters the cells through the Spike mediated interaction with the ECD domain of the ACE2 cell receptor. A recombinant fusion protein (RBD of Spike protein and ECD of membrane protein) can a great tool to investigate this interaction. Learn more about the purified recombinant Spike Protein (S)
Recently several SARS-CoV-2 variants with mutation in the spike (S)-protein has been identified. Of all the variants analyzed, variants carrying D614G mutation was significantly more infectious than others. Some of the variants with amino acid change in receptor binding domain (RBD) were less infectious and some were resistant to neutralizing antibodies. Understanding the impact of these mutations is of great value for development of vaccines and therapeutic antibodies.
SARS-CoV-2 RBD Neutralizing Monoclonal Antibody:SARS-Cov-2 RBD neutralizing antibody (Cat# TA814512) inhibits human ACE2 protein (Cat# TP701115) binding to SARS-CoV-2 RBD protein TP701119). The IC50 is typically 0.91 nM.
ACE-2 receptor: ACE-2 is the host cell receptor responsible for mediating infection by SARS-CoV-2.
Tools for analyzing ACE-2 protein
- Human Recombinant Protein Angiotensin Converting Enzyme (ACE2)
- ACE-2 specific Mouse Monoclonal Antibody
- ACE-2 Human ORF clones
Find out more about the additional ACE-2 specific tools here
Furin: It is a protease present in many human organs that recognizes and activates a specific site on the SARS-CoV-2 Spike protein, thus facilitating a tighter binding to the ACE-2 receptor and might play a role in the higher infection rate .
How can you analyze Furin? Learn about the tools for analyzing Furin.