ACE2 (NM_021804) Human Recombinant Protein (Angiotensin Converting Enzyme 2)

CAT#: TP701115

Purified recombinant Human angiotensin I converting enzyme (peptidyl-dipeptidase A) 2 (ACE2), extracellular domain of ACE spanning 1-615 residues, with C-terminal Human FC tag, expressed in HEK293.

Product Images

Purified recombinant protein ACE2 protein was analyzed by SDS-PAGE gel and Coomossie Blue Staining.

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  Binding activity measured by ELISA: Immobilized SARS-CoV-2 Spike Membrane Fusion Protein (RBD of S-Protein and Virion Surface Domain of M-Protein with His Tag; Cat.No. TP701119) at 2 µg/mL (100 µL/well) can bind to Human ACE2 Fc Tag (Cat.No. TP701115), the EC50 of the human ACE2 protein is 10-20 ng/ml.

Specifications

Product Data

• Species: Human
• Expression Host: HEK293
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  MDWIWRILFL VGAATGAHSQ STIEEQAKTF LDKFNHEAED LFYQSSLASW NYNTNITEEN
  VQNMNNAGDK WSAFLKEQST LAQMYPLQEI QNLTVKLQLQ ALQQNGSSVL SEDKSKRLNT
  ILNTMSTIYS TGKVCNPDNP QECLLLEPGL NEIMANSLDY NERLWAWESW RSEVGKQLRP
  LYEEYVVLKN EMARANHYED YGDYWRGDYE VNGVDGYDYS RGQLIEDVEH TFEEIKPLYE
  HLHAYVRAKL MNAYPSYISP IGCLPAHLLG DMWGRFWTNL YSLTVPFGQK PNIDVTDAMV
  DQAWDAQRIF KEAEKFFVSV GLPNMTQGFW ENSMLTDPGN VQKAVCHPTA WDLGKGDFRI
  LMCTKVTMDD FLTAHHEMGH IQYDMAYAAQ PFLLRNGANE GFHEAVGEIM SLSAATPKHL
  KSIGLLSPDF QEDNETEINF LLKQALTIVG TLPFTYMLEK WRWMVFKGEI PKDQWMKKWW
  EMKREIVGVV EPVPHDETYC DPASLFHVSN DYSFIRYYTR TLYQFQFQEA LCQAAKHEGP
  LHKCDISNST EAGQKLFNML RLGKSEPWTL ALENVVGAKN MNVRPLLNYF EPLFTWLKDQ
  NKNSFVGWST DWSPYADEPK SCDKTHTCPP CPAPELLGGP SVFLFPPKPK DTLMISRTPE
  VTCVVVDVSH EDPEVKFNWY VDGVEVHNAK TKPREEQYNS TYRVVSVLTV LHQDWLNGKE
  YKCKVSNKAL PAPIEKTISK AKGQPREPQV YTLPPSRDEL TKNQVSLTCL VKGFYPSDIA
  VEWESNGQPE NNYKTTPPVL DSDGSFFLYS KLTVDKSRWQ QGNVFSCSVM HEALHNHYTQ
  KSLSLSPGK
• Tag: C-FC
• Predicted MW: 95.3 kDa
• Concentration: >50 ug/mL as determined by Bradford protein assay method.
• Purity: > 90% as determined by SDS-PAGE and Coomassie blue staining
• Buffer: PBS, 10% glycerol.
• Storage: Store at -80°C.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.

Reference Data

• RefSeq: NP_068576
• Locus ID: 59272
• UniProt ID: Q9BYF1
• Cytogenetics: Xp22.2
• Synonyms: ACEH
• Summary: The protein encoded by this gene belongs to the angiotensin-converting enzyme family of dipeptidyl carboxydipeptidases and has considerable homology to human angiotensin 1 converting enzyme. This secreted protein catalyzes the cleavage of angiotensin I into angiotensin 1-9, and angiotensin II into the vasodilator angiotensin 1-7. The organ- and cell-specific expression of this gene suggests that it may play a role in the regulation of cardiovascular and renal function, as well as fertility. In addition, the encoded protein is a functional receptor for the spike glycoprotein of the human coronaviruses SARS and HCoV-NL63. [provided by RefSeq, Jul 2008]
• Protein Families: Druggable Genome, Secreted Protein, Transmembrane
• Protein Pathways: Renin-angiotensin system