ORF Cloning Vectors

The same ORF insert in multiple context for diverse applications

All TrueORF inserts are housed in a pCMV6-Entry vector and can be easily shuttled via PrecisonShuttle, a simple 'cut-and-paste' procedure, into any of the destination vectors. With the wide selection of destination vectors, TrueORF clones are ready-to-use for many downstream protein studies.

The destination vectors offer choices of:

  • Epitope tags for protein purification and detection
  • Fluorescent protein tags for visualization
  • Various drug selection markers for stable cell line establishment
  • Lentiviral, and AAV based expression vectors
  • New: Tet-on tetracycline-inducible vectors

The vectors could be ordered as:

Lentiviral Vectors

OriGene now offers multiple Lentiviral vectors. Easy shuttling of TrueORF insert. Click the Lentiviral tab below

Epitope tagging

FP tagging

TrueORF Photo gallery of the fluorescent-tagged proteins generated by OriGene with TrueORF clones

Selection markers

Lenti / AAV

Inducible vectors

Bacterial expression

The new E.coli destination vectors expand the utility of 37,000 TrueORF clones for protein production in bacteria.
Expression Validation Product Manual

Peptide sequence of the DDK-tag (Flag®): N-DYKDDDDK-C
Flag® is a registered trademark of Sigma-Aldrich