Over-Expression Cell Lysate Controls (OEL Controls)

A validated positive control for Western Blot, ELISA

Full length human proteins as positive controls for antibody validation

OriGene offers a large collection of tagged over-expression lysates, a cost-effective assay standard for Western blot, ELISA, and other antibody assays.

Features include

• 20,000 over-expression lysates of full length human proteins
• Covering 80% of the human proteome
• Expressed in mammalian HEK293T cells
• C-terminal myc-DDK (FLAG) tag for easy expression detection and isolation

? Search for your over-expression Lysate (browse all)

Improved anti-DDK antibody

We strive to continuously improve our products to ensure you have the best tools for your research. Our new anti-DDK antibody (TA592569) has undergone?rigorous validation?and, at each step, has given better results.?

Features of our new anti-DDK antibody:

• Improved sensitivity (5x -10x depending on your protein expression)
• Minimal background
• Validated for multiple applications: WB, IHC, IF, FC, ELISA, IP

Easily detect your target of interest from OriGene Lysates using our new, highly specific anti-DDK antibody. Works with both N and C-terminus tagged proteins.

TrueORF cDNA clones With Myc-DDK (FLAG) tags

Transfection into HEK293T

Over-expression Lysates of Full Length Human Proteins

• Positive control for antibody validation including western blot, ELISA, and etc.
• Protein functional assays
• Immunoprecipitation, protein-protein interaction

Immunoblot analysis of 6 different C-terminal Myc/DDK tagged over-expression lysates with TA592569 at 1:3000.
1.CST4, 2.LSM1, 3.ALDOB, 4.ALDH3A1, 5.PLK1, 6.METTL16

Immunoblot analysis of 5 different N-terminal DDK tagged over-expression lysates with TA592569 at 1:3000.
1.Trim29 fragment (1-163aa), 2.SNCA, 3.SNCB, 4.TNFA, 5.Trim29 fragment (164-449aa)