ELISA Kits

What is ELISA (enzyme-linked immunosorbent assay)?

Enzyme-linked immunosorbent assay, also known as Enzyme-Immunoassay (EIA) is a biochemical technique to detect and quantify the presence of antigens, hormones, and antibodies. In an ELISA, the antigen (target macromolecule) from the sample to be tested is immobilized on a solid surface (microplate) and then a matching antibody is applied which is linked to a reporter enzyme. Detection is accomplished by measuring the activity of the reporter enzyme via incubation with the appropriate substrate to produce a measurable product.

OriGene offers hundreds of ELISA kits in immunometric assay formats. These kits allow specific, quantitative measurements of disease-related proteins, including cytokines, chemokines and signaling targets.

ELISA Kits
ELISA Reagents
ELISA protocol

Types of ELISA

Direct ELISA - In this method, the ELISA plate is coated with antigens, the conjugated detection antibody then binds to the target protein. OriGene produce high quality, ELISA applicable, and HRP/AP/biotin conjugated primary antibodies for you to choose to construct your assay.
Direct ELISA
Indirect ELISA - It is a two-step detection method. Similar to direct ELISA, antigen is immobilized to the plate. The primary antibody specific for the antigen binds the target, and a secondary antibody binds to the primary antibody for detection. OriGene produce high quality, ELISA applicable primary or HRP/AP/biotin conjugated secondary antibodies for you to choose to construct your assay.
Indirect ELISA
Sandwich ELISA - This method uses two types of antibodies: 1) Capture antibody 2) Detection antibody. The capture antibody is coated on the plate; this binds to the antigen which in turn binds to the primary antibody for detection. OriGene offer sandwich ELISA kits in either conventional or Luminex assay platform for wide-ranged selected targets to meet your research purpose.
Sandwich ELISA
? Search for your ELISA (browse all)

Recent Citations using our ELISA Kits

1. Corinthian Currants Supplementation Restores Serum Polar Phenolic Compounds, Reduces IL-1beta, and Exerts Beneficial Effects on Gut Microbiota in the Streptozotocin-Induced Type-1 Diabetic Rat

Published in Metabolites, 2023 Mar, using EA100168 		PubMed: 36984855

2. Effect of Smoke Exposure on Gene Expression in Bone Healing around Implants Coated with Nanohydroxyapatite

Published in Nanomaterials, 2022 Oct, using EA100902 		PubMed: 36364513

3. Primary Human Trophoblasts Mimic the Preeclampsia Phenotype after Acute Hypoxia–Reoxygenation Insult

Published in Cells, 2022 Jun, using EA100379 		PubMed: 35741027

See all ?

Assay Kit Resources