Melanoma Antigen (MAGE) Family - More than a Cancer Cell Marker

The melanoma antigen (MAGE) protein family, containing a ~180-amino-acid MAGE homology domain (MHD), are conserved in eukaryotes and have ~40 members in human and mouse. MAGEA1, the founder member of this family, was first identified as a cancer cell marker in melanoma, and soon after, it was found having restricted expression in male gonad, a mammalian body sites with immune privilege. Due to the antigenic potential, male germline expression, and aberrantly re-activated in cancer cells, two third of MAGE proteins have been categorizes as cancer-testis antigens (CTA).

OriGene offers a range of tools for analyzing MAGE Protein Family. Click below for details

cDNA Clones (549)

Recombinant Proteins (202)

Monoclonal Antibodies (196)

Adenocarcinoma of Human colon tissue stained with anti-MAGEA3 mouse monoclonal antibody (TA800828)

Carcinoma of Human bladder tissue stained with anti-MAGEA4 mouse monoclonal antibody (TA505362)

Carcinoma of Human bladder tissue stained with anti-MAGEB1 mouse monoclonal antibody (TA800473)

Adenocarcinoma of Human ovary tissue using anti-MAGEB18 mouse monoclonal antibody (TA502633)

MAGE Proteins: Normal function and Implication in Diseases

The physiological functions of MAGE proteins are diverse. For example, MAGEAs enhance the resistance to the genotoxic stress during spermatogenesis. MAGEB2 maintains thermo-sensitive spermatogenesis by suppressing stress granule formation. MAGED1 regulates neuronal apoptosis during neurogenesis and plays essential roles in various brain functions, such as depression, memory, behavior, and circadian rhythm. MAGED2, mutations of which cause X-linked polyhydramnios with prematurity and a transient form of antenatal Bartter's syndrome, regulates expression and function of Na-K-Cl cotransporter (NKCC2) and Na-Cl cotransporter (NCC). Children with MAGEG1 mutations suffered from lung disease immunodeficiency and chromosome breakage syndrome. MAGEL2 is implicated in various human genetic disorders, e.g. Prader-Willi syndrome and Schaaf-Yang syndrome.

MAGE Proteins in Cancers

MAGE proteins have widespread expression in different cancers and are usually poor prognostic markers for cancer patients. As convincing cancer cell markers, MAGE proteins (e.g. MAGEA1, A3, A4, A6, A10, A12, C2) have been immuno-targeted in many clinical trials for cancer. However, myriad evidence indicates that MAGE proteins also play an active role in oncogenicity, tumor progression, and cancer cell survival. For example, MAGEA3 can suppresses cancer cell apoptosis through directly interacting with TP53. MAGEA3 can also complex with TRIM28 to ubiquitinate and degrade TP53 or AMPKα, a master sensor/regulator of cell energy homeostasis, which is commonly suppressed in early-stage cancers. MAGEA11 complexed with HUWE1 performs ubiquitin E3 ligase activity to degrade PCF11, a mRNA 3' end processing complex protein, resulting in 3' UTR shortening, a common feature of most cancer cells. MAGEF1-NSMCE1 E3 ubiquitin ligases ubiquitinate and degrade MMS19, an essential protein in cytosolic iron-sulfur (Fe-S) cluster assembly (CIA) pathway, leading to defects in DNA repair and increasing mutation burden in cancer cells. MAGE proteins should be considered as new therapeutic targets for cancers.

MAGEA Antibody Panel

• MAGEA1: AM32863PU-T

  20 ug

  MAGE 1 (MAGEA1) mouse monoclonal antibody, clone MA454, Purified

• MAGEA2: AP13129PU-N

  400 ul

  MAGEA2 (MAGEA2B) (N-term) rabbit polyclonal antibody, Purified

• MAGEA3: TA800826S

  30 ul

  MAGEA3 mouse monoclonal antibody,clone OTI1H1

• MAGEA4: TA505362S

  30 ul

  MAGEA4 mouse monoclonal antibody, clone OTI1F9 (formerly 1F9)

• MAGEA5: TA339350

  100 ul

  Rabbit Polyclonal Anti-MAGEA5 Antibody

• MAGEA9: TA800839S

  30 ul

  MAGEA9 mouse monoclonal antibody,clone OTI1D8

• MAGEA10: TA350148

  100 ul

  Rabbit Polyclonal Anti-MAGEA10 Antibody

• MAGEA11: TA365791

  100 ul

  MAGEA11 rabbit polyclonal antibody

• MAGEA12: TA357642

  100 ul

  MAGEA12 Antibody - N-terminal region

OriGene offers a range of tools for analyzing MAGE Protein Family. Click the boxes for details

MAGE in Clinical Trial

A1 A3 A4 A6 A10 A12 C2

MAGE - Ubiquitin E3 Ligase

TRIM28 RAD18 HUWE1 SKIP2 LNX1 RBX1 PJA1 NSMCE1 TRIM27 RNF41

MAGE Family

A1 A2 A3 A4 A5 A6 A8 A9 A10 A11 A12 B1 B2 B3 B4 B5 B6 B10 B16 B17 B18 C1 C2 C3 D1 D2 D3 D4 E1 E2 F1 G1 H1 L2 NDN

Gene	CTA*	Function	Associated proteins	Activity	Target	Pathway	Reference (PubMed ID)
MAGEA2	x	transcription	TP53, HDAC3	transcription repressor	CDKN1A	cell cycle regulation	16847267
		transcription		chromatin binding blocker	TP53	tumor suppression	21056992
		protein degradation	TRIM28	E3 ubiquitin ligase	TP53	tumor suppression	20864041
MAGEA3/6	x	protein degradation	TRIM28	E3 ubiquitin ligase	AMPK alpha (PRKAA1)	cell metabolism	25679763
		protein degradation	TRIM28	E3 ubiquitin ligase	ZNF382	cell cycle regulation	21876767
		protein degradation	TRIM28	E3 ubiquitin ligase	FBP1	cell metabolism	28394358
		unknown		negetive regulator	BIRC5	apoptosis	34166362
MAGEA4	x	protein degradation	RAD18	E3 ubiquitin ligase	PCNA	Trans-lesion synthesis (TLS)	27377895
MAGEA11	x	protein degradation	HUWE1	E3 ubiquitin ligase	PCF11	mRNA alternative polyadenylation (APA)	31980388
		Transcription	AR, EP300, NCOA2	transcription activator	KLK3	androgen response	20448036
		Transcription	PGR (B isoform)	transcription activator	FKBP5	progesteron response	22891251
		protein degradation/ stabilization	SKP2 (SCF complex)	E3 ubiquitin ligase	CCNA2, E2F1, RBL1	cell cycle regulation	27720894
MAGEB2	x	Translation		translation repressor (DDX5 antagonist)	G3BP1	stress granule formation	32692974
		Transcription	HDAC1	transcription activator	E2F1	cell cycle regulation	26468294
		Transcription		rRNA transcription pre-initiation complex	UBTF	ribosome biogenesis	33741433
MAGEB18	x	protein degradation	LNX1	E3 ubiquitin ligase		unknown	20864041
MAGEC2	x	protein degradation	TRIM28	E3 ubiquitin ligase	AMPK alpha (PRKAA1)	cell metabolism	25679763
		protein degradation	TRIM28	E3 ubiquitin ligase	TP53	tumor suppression	20864041
		protein degradation	TRIM28	E3 ubiquitin ligase	ZNF382	cell cycle regulation	21876767
		protein degradation		ubiquitination	ZMYND11	tumor suppression	24866244
		protein stabilization		polyubiquitination inhibition	STAT3	cancer metastasis	27775077
		protein stabilization	RBX1, Skp1-Cullin1-F box protein (SCF)	E3 ubiquitin ligase inhibition	CCNE1	cell cycle regulation	26540345
		protein degradation	TRIM28	E3 ubiquitin ligase	FBP1	cell metabolism	28394358
MAGED1		Signaling	NGFR	signaling adaptor	JNK	neuronal apoptosis	12376548
		Signaling	UNC5A	signaling adaptor		neuronal apoptosis	12598531
		protein degradation		pro-apoptotic protein	XIAP	neuronal apoptosis	11546791
		protein degradation		pro-apoptotic protein	AATF	neuronal apoptosis	17488777
		Signaling	TAK1, TAB1, XIAP	signaling complex	MAPK14	apoptosis	16107717
		Transcription		transcription coactivator	DLX5	cell differentiation	11084035
		protein degradation	PJA1	E3 ubiquitin ligase		unkown	20864041
		transcription	NDN	transcription factor modulator	MSX1	cell differentiation	15272023
		protein degradation		ubiquitination	SLC6A4	neurotransmitter serotonin retrieval	22457503
		transcription	RORA	transcription coactivator	ARNTL	circadian rhythm	20300063
		transcription	CREB1	transcription coactivator	BDNF, ATF4, NRXN1, GRIN2B, EGR1	synaptic plasticity	24700102
MAGED2		unkown	NCL	unkown		cell cycle: G1/S transition	29371029
		transcription	TP53	transcription factor modulator		apoptosis	17912449
		protein stablization	GNAS, DNAJB1	unkown	SLC12A1, SLC12A3	Bartter's syndrome	27120771
MAGEF1		protein degradation	NSMCE1	E3 ubiquitin ligase	MMS19	cytosolic iron-sulfur (Fe-S) cluster assembly (CIA) pathway	29225034
MAGEG1 (NDNL2, NSMCE3)		protein complex	SMC5/6 complexes	DNA replication fork stabilizer		DNA replication and repair	17005570
		protein degradation	NSMCE1	E3 ubiquitin ligase		unknown	20864041
MAGEH1		unkown	GADD45G	caspases activation		apoptosis	34788311
MAGEL2		protein degradation		E3 ubiquitin ligase	CRY1	circadian rhythm	32315313
		mRNA processing		mRNA m6A methylation	YTHDF2	RNA metabolism	34265304
		signaling	TRIM27, USP7	E3 ubiquitin ligase	WASHC1	endosomal protein recycling	26365382
		signaling	NDN, RNF41, USP8	E3 ubiquitin ligase	LEPR	endosomal protein recycling	28973533
NDN		transcription	SIRT1	deacetylase	TP53	neuronal apoptosis	18753379

*Cancer Testis Antigen

References

• Florke Gee et al. 2020 Nov 20;295(47):16121-16155. doi: 10.1074/jbc.REV120.008029
• Hasegawa and Yoshikawa. 2008 Aug 27;28(35):8772-84. doi: 10.1523/JNEUROSCI.3052-08.2008
• Shin et al. 2021 Nov 17;16(11):e0260135. doi: 10.1371/journal.pone.0260135
• Sanderson et al. 2021 Aug;297(2):100959. doi: 10.1016/j.jbc.2021.100959
• Ladelfa et al. 2021 Jun;1868(7):119015. doi: 10.1016/j.bbamcr.2021.119015
• Craig et al. 2021 Jun 24;17(6):e1009589. doi: 10.1371/journal.pgen.1009589