Stable Cell Line Development Service

Why go through the tedious process of stable cell line development?

Pass your project to our experts and utilize OriGene's expertise.

What We Offer:

  • Tet-on Inducible expression
  • Over Expression (ORF)
  • Knockdown (shRNA)
  • Knock-in (CRISPR)
  • Knockout (CRISPR)

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SL500077SL500075
Figure 1: Validation data of HEK293T (left, sku # SL500075 and HEK293 (right, sku # SL500078) cell lines expressing GFP

Case Studies: Over-expression in Stable Cell Lines

A human gene (Target X) was cloned into an expression vector (PS100024) and transfected into mammalian cells. Single colonies were established after 4 weeks of hygromycin drug selection. Western blot using a gene-specific antibody for Target X and ?-actin antibody (OriGene, TA811000) demonstrates over-expression of Target X in Clones 1, and at a higher level in Clone 2 (OriGene internal data).

A human gene (Target Y) was cloned into an expression vector (PS100024) and transfected into mammalian cells. Single colonies were established after 4 weeks of hygromycin drug selection. Western blot using a gene-specific antibody for Target Y and ?-actin antibody (OriGene, TA811000) demonstrates over-expression of Target Y in the stable cell line (OriGene internal data).

Service Highlights:

  • Fast turnaround time: 2-3 months
  • Choose from OriGene's extensive ORF clone collection or provide your own expression cassette
  • Rigorous and customizable QC validation process
  • Professional, Global Technical Support

Cell Line Development Project Design

At OriGene, you have the power to customize every step of the stable cell line development process. From vector selection to final delivery, we offer a spectrum of options designed to cater to your specific needs.

Vector Selection
  • Subcloning
  • Gene Synthesis
  • Sequencing/ Expression Validation (TrueORF Gold)
Cell Line Development
  • Cell line selection
  • Viral/non-viral transfection options
Cell Line Validation
  • Western Blot
  • Mycoplasma Detection
  • Custom options available
Delivery
  • 2-3 months

GFP Controls for your Over-expression Experiments:

Name Description Catalog #
GFP Over-expression Stable Cell Line - HEK293T GFP Over-expression Stable Cell Line - HEK293T, 1 vial, 3 x 10^6 cells/vial, Cryopreserved SL500075
GFP Over-expression Stable Cell Line - Hela GFP Over-expression Stable Cell Line - Hela, 1 vial, 3 x 10^6 cells/vial, Cryopreserved SL500076
GFP Over-expression Stable Cell Line - MDA-MB-231 GFP Over-expression Stable Cell Line - MDA-MB-231, 1 vial, 3 x 10^6 cells/vial, Cryopreserved SL500077
GFP Over-expression Stable Cell Line - HEK293 GFP Over-expression Stable Cell Line - HEK293, 1 vial, 3 x 10^6 cells/vial, Cryopreserved SL500078