Stable Cell Line Development Service

Why go through the tedious process of stable cell line development?

Pass your project to our experts and utilize OriGene's expertise.

What We Offer:

  • Tet-on Inducible expression
  • Over Expression (ORF)
    • Inducible
    • Constitutive
  • Knockdown (shRNA)
    • Inducible
    • Constitutive
  • Knock-in (CRISPR)
  • Knockout (CRISPR)

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SL500077 SL500075
Fig. 1. Validation data of HEK293T (left, sku # SL500075 and HEK293 (right, sku # SL500078) cell lines expressing GFP

Inducible Lentiviral Particles for Overexpression
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Fig. 2. Dose-dependent GFP expression in HEK293T cells transduced with inducible lentiviral GFP particles and treated with varied Dox concentrations for 5 days (original magnification, 10x). High GFP expression was observed by the treatment of 10 ?g/mL Dox.

Service Highlights:

  • Fast turnaround time: 2-3 months
  • Choose from OriGene's extensive ORF clone collection or provide your own expression cassette
  • Rigorous and customizable QC validation process
  • Professional, Global Technical Support

Cell Line Development Project Design

At OriGene, you have the power to customize every step of the stable cell line development process. From vector selection to final delivery, we offer a spectrum of options designed to cater to your specific needs.

Vector Selection
  • Subcloning
  • Gene Synthesis
  • Sequencing/ Expression Validation (TrueORF Gold)
  • Inducible vs. Constitutive
Cell Line Development
  • Cell line selection
  • Viral/non-viral transfection options
Cell Line Validation
  • Western Blot
  • Mycoplasma Detection
  • Custom options available
Delivery
  • Constitutive: 2-3 months
  • Inducible: 3-4 months

Inducible Stable Cell Line Development

Inducible expression is a valuable tool for functional analysis of assay systems with toxic targets. OriGene's All-in-one Tet-On system is a new and improved version of the original Tet-On systems, designed to significantly stimulate expression of the downstream gene of interest (GOI). Click here to learn more about our inducible vectors.

Stable cell lines
Figure 2. Human GPR37 cDNA gene was cloned into a mammalian Tet-On inducible vector (PS100133). HEK293T cells were transfected with a PS100133-GPR37 construct (0.5 ?g DNA/well of a 12-well plate). Single stable HEK293T colonies were established after 4 weeks of hygromycin drug selection at 200 ?g/mL and then treated with or without Dox at 5 ?g/mL for 3 days. High GFP expression was observed by the treatment of Dox for 3 days.

GFP Controls for your Over-expression Experiments:

Name Description Catalog #
GFP Over-expression Stable Cell Line - HEK293T GFP Over-expression Stable Cell Line - HEK293T, 1 vial, 3 x 10^6 cells/vial, Cryopreserved SL500075
GFP Over-expression Stable Cell Line - Hela GFP Over-expression Stable Cell Line - Hela, 1 vial, 3 x 10^6 cells/vial, Cryopreserved SL500076
GFP Over-expression Stable Cell Line - MDA-MB-231 GFP Over-expression Stable Cell Line - MDA-MB-231, 1 vial, 3 x 10^6 cells/vial, Cryopreserved SL500077
GFP Over-expression Stable Cell Line - HEK293 GFP Over-expression Stable Cell Line - HEK293, 1 vial, 3 x 10^6 cells/vial, Cryopreserved SL500078