MicroRNA Expression Plasmids

MicroRNAs (miRNAs) are naturally occurring non-coding RNA molecules (containing 21-23 nucleotides) that function in RNA silencing and post-transcriptional regulation of gene expression.

OriGene provides clones that facilitate over-expression of the miRNA of your choice. A region of ~600bp encompassing each miRNA is cloned downstream of a CMV promoter. Upon transfection, the cellular machinery will process the CMV-driven expression of miRNA precursor into mature miRNA, and cellular function can be analyzed.

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MicroRNA Processing Diagram

Key Features

  • Genome-wide miRNA coverage (miRBase 20.0): 1829 human, 1160 mouse, 436 rat
  • GFP reporter for transfection monitoring
  • Neomycin selection marker for stable cell establishment
  • 3' UTR reporter clones available for miRNA target validation
  • Cloning Vector: pCMV-MIR

Bulk Order Options

The complete set of the miRNA expression plasmids is available upon inquiry. Any purchase of over 10 miRNA plasmids is qualified for discount. Please contact techsupport@origene.com for more details.

Description List
Mouse miRNA expression plasmid set (486 vectors, 10ug each in 2-D bar coded tubes)
Human miRNA expression plasmid set (646 vectors, 10ug each in 2-D bar coded tubes)
Mouse miRNA expression plasmid set (486 vectors, 2ug each in 96-well plates)
Human miRNA expression plasmid set (646 vectors, 2ug each in 96-well plates)

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Recent Citations using our MicroRNA Expression Plasmids

1. miR-155-5p can be involved in acquisition of osseointegration on titanium surface.

Published in In vitro cellular & developmental biology. Animal, 2022 Oct, using SC400793 		PubMed: 36053380

2. microRNA ‐29a Regulates ADAM12 Through Direct Interaction With ADAM12 mRNA and Modulates Postischemic Perfusion Recovery

Published in Journal of the American Heart Association: Cardiovascular and Cerebrovascular Disease, 2022 Aug, using SC400963 		PubMed: 35946473

3. miR-637 Prevents Glioblastoma Progression by Interrupting ZEB2/WNT/β-catenin Cascades.

Published in Cellular and molecular neurobiology, 2022 Aug, using SC400614 		PubMed: 34047878

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RNAi Resources