3’-UTR Clones
Assay-ready Luc-UTR constructs for 20,000 human genes
The 3’-UTR plasmids provide a convenient solution for quantitative assessment of the inhibitory effect between miRNAs and their potential target genes. The 3’-UTR sequence of a gene of interest was cloned downstream of the firefly luciferase gene. The chimeric transcript level is regulated by its interaction with miRNA(s), which results in varied luciferase activity quantifiable by a colorometric assay
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Interaction between miRNA and UTR Reduced luciferase expression |
No interaction between miRNA and UTR No effect on luciferase expression |
5,000 Human ORF clones at $98 each
16,000 Mouse ORF clones starting at $48 each
Features:- Genome wide coverage (>20,000 human gene).
- Firefly luciferase as the easy-to-assay reporter
- RFP as reporter for transfection monitoring
- High sensitivity from IRES-driven translation of the expression cassette
- 3'-UTRs clones longer than 2kb can be ordered through gene synthesis. Make sure to select pMirTarget as the cloning vector.