MAGE 1 (MAGEA1) Mouse Monoclonal Antibody [Clone ID: MA454]

CAT#: AM32863PU-T

MAGE 1 (MAGEA1) mouse monoclonal antibody, clone MA454, Purified


USD 240.00

2 Weeks*

Size
    • 20 ug

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Specifications

Product Data
Clone Name MA454
Applications FC, IF, IHC, IP, WB
Recommended Dilution ELISA (Use Antibody without BSA For coating). 
Flow Cytometry: 0.5-1 µg/106 cells. 
Immunofluorescence: 1-2 µg/ml. 
Western Blot: 0.5-1 µg/ml. 
Immunoprecipitation: 1-2 µg/500 µg protein lysate. 
Immunohistochemistry on Frozen and Formallin-Fixed Paraffin Sections: 0.5-1 µg/ml for 30 minutes at RT.
Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. 
Positive Control: Melanoma cell lines. Melanomas, gliomas, neuroblastoma, non-small cell lung cancer, breast, gastric, colorectal, ovarian, and renal cell carcinomas.
Reactivities Canine, Human, Rat
Host Mouse
Isotype IgG1
Clonality Monoclonal
Immunogen Human MAGE-A1 full length recombinant protein.
Specificity This Monoclonal MA454 antibody recognizes a protein of 42-46kDa, identified as MAGE-1 and does not cross-react with MAGE-2, -3, -4, -6 -9, -10, -or -12 protein.
Cellular Localization: Cytoplasmic.
Formulation 10mM PBS
State: Purified
State: Liquid purified IgG fraction from Bioreactor Concentrate
Stabilizer: 0.05% BSA
Preservative: 0.05% Sodium Azide
Concentration lot specific
Purification Protein A/G Chromatography
Storage Store undiluted at 2-8°C.
Stability Shelf life: one year from despatch.
Predicted Protein Size 42-46 kDa
Gene Name MAGE family member A1
Background MAGE-1 is a cancer testis antigen (CTA) expressing in a variety of human cancers (Pereira CM et al., 2012). It is a human melanoma antigen recognized by cytolytic T lymphocytes. MAGE-1 induces a tumor specific immune response so it is thought to be a potential therapeutic target for cancer immunotherapy (Ma ZY et al., 2002). The gene for MAGE-1 belongs to a family of 12 genes known as MAGE-A cluster located on the X chromosome in region q28 (Laduron S et al., 2004). This relies primarily on DNA methylation for repression in somatic tissues but in many types of tumors, the promoter of these genes becomes demethylated and transcription becomes activated (Loriot A et al., 2006). Monoclonal antibody aganist a recombinant feline melanoma antigen 1 (fMAGE-1) has been generated and is effective against feline lymphoma cell lines and tumor tissues (Ma ZY et al., 2002).
Synonyms MAGE 1, MAGE1, Melanoma-associated antigen 1, MAGEA1, MAGE1A
Reference Data

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.