SARS-CoV-2 Spike-Membrane Recombinant Fusion Protein (Bioactivity Verified)

CAT#: TP701119

Purified recombinant fusion protein that includes the RBD of SARS-CoV-2 Spike protein (S-Protein) and Virion Surface Domain of Membrane Protein (M-protein) with C-terminal HIS-tag

Product Images

Purified recombinant protein S-M protein was analyzed by SDS-PAGE gel and Coomossie Blue Staining.

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  Binding activity measured by ELISA: Immobilized SARS-CoV-2 Spike Membrane Fusion Protein (RBD of S-Protein and Virion Surface Domain of M-Protein with His Tag; Cat.No. TP701119) at 2 µg/mL (100 µL/well) can bind to Human ACE2 Fc Tag (Cat.No. TP701115), the EC50 of the human ACE2 protein is 10-20 ng/ml.

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  ELISA plates were coated with anti-HIS (Cat# TA150088) as capture antibody. HIS-tagged SARS-CoV-2 Spike-membrane recombinant fusion protein (Cat# TP701119) was then added as substrate. Human anti-S antibody (Cat# TA190325) with concentration of 0.05 ug/ml against SARS-CoV-2 Spike protein RBD was applied for detection. HRP assay was used for readout. Coating with anti-DDK antibody (Cat# TA50011-100) was served as negative control.

Specifications

Product Data

• Species: SARS-CoV-2
• Expression Host: HEK293
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  MALWIDRMQL LSCIALSLAL VTNSAKGIYQ TSNFRVQPTE SIVRFPNITN LCPFGEVFNA
  TRFASVYAWN RKRISNCVAD YSVLYNSASF STFKCYGVSP TKLNDLCFTN VYADSFVIRG
  DEVRQIAPGQ TGKIADYNYK LPDDFTGCVI AWNSNNLDSK VGGNYNYLYR LFRKSNLKPF
  ERDISTEIYQ AGSTPCNGVE GFNCYFPLQS YGFQPTNGVG YQPYRVVVLS FELLHAPATV
  CGPKKSTNLV KNKCVNFNFN GLTGTGVLTE SNKKFLGGGS GGGSMADSNG TITVEELKKH
  HHHHH
• Tag: C-HIS
• Predicted MW: 34 kDa
• Concentration: >50 ug/mL as determined by Bradford protein assay method.
• Purity: > 90% as determined by SDS-PAGE and Coomassie blue staining
• Buffer: PBS, 10% glycerol.
• Bioactivity: Binding of SARS-CoV-2 S-M Fusion Protein to Human ACE2: 2 ug/ml of SARS-CoV-2 Spike and Membrane viral fusion protein (gel insert) was immobilized on an ELISA plate and its ability to bind to Human ACE2 was measured. The EC50 was between 10-20 ng/ml.
• Storage: Store at -80°C.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.