EBV-VCA IgM ELISA Kit
USD 506.00
3 Weeks*
Size
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Specifications
Product Data | |
Format | 96-well strip plate |
Assay Type | Solid Phase Sandwich ELISA |
Assay Length | 3 hours |
Signal | Colorimetric |
Sample Type | Cell culture supernatant, serum, plasma |
Sample Volume | 10 ul |
Specificity | Natural and recombinant human EBV-VCA IgM |
Reactivities | Human |
Interference | No significant interference observed with available related molecules. |
Components | See the product information for more details |
Background | The Epstein-Barr Virus (VCA) IgM ELISA Kit is a solid phase enzyme-linked immunosorbent assay (ELISA). Specimen samples are diluted with Sample Diluent and additionally incubated with IgG-RF-Sorbent, containing hyper-immune anti-human IgG-class antibody to eliminate competitive inhibition from specific IgG and to remove rheumatoid factors. This pretreatment avoids errors in results. Microtiter wells as a solid phase are coated with inactivated Epstein-Barr viral capsid antigen. Pretreated sample specimens and ready-for-use controls are pipetted into these wells. During incubation Epstein-Barr viral capsid antigen-specific antibodies of specimens where analyte is present and controls are bound to the immobilized antigens. After a washing step to remove unbound sample and control material horseradish peroxidase conjugated anti-human IgM antibodies are dispensed into the wells. During a second incubation this anti IgM conjugate binds specifically to IgM antibodies resulting in the formation of enzyme-linked immune complexes. After a second washing step to remove unbound conjugate the immune complexes formed (in cases where analyte is found) are detected by incubation with TMB substrate and development of a blue color. The blue color turns into yellow by stopping the enzymatic indicator reaction with sulfuric acid. The intensity of this color is directly proportional to the amount of Epstein-Barr viral capsid antigen-specific IgM antibody in the sample specimen. Absorbance at 450 nm is read using an ELISA microtiter plate reader. |
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.