Fasciola hepatica IgG ELISA Kit

CAT#: EA101104

Fasciola hepatica IgG ELISA Kit

Specifications

Product Data

• Format: 96-well strip plate
• Assay Type: Solid Phase Sandwich ELISA
• Assay Length: 3 hours
• Signal: Colorimetric
• Sample Type: Cell culture supernatant, serum, plasma
• Sample Volume: 10 ul
• Specificity: Natural and recombinant human Fasciola hepatica IgG
• Reactivities: Human
• Interference: No significant interference observed with available related molecules.
• Components: See the product information for more details
• Background: The Fasciola IgG ELISA Kit is a solid phase enzyme-linked immunosorbent assay (ELISA). Microtiter wells as a solid phase are coated with Fasciola antigen from adult liver fluke. Diluted sample specimens and ready-for-use controls are pipetted into these wells. During incubation Fasciola-specific antibodies of positive specimens and controls are bound to the immobilized antigens. After a washing step to remove unbound sample and control material horseradish peroxidase conjugated anti-human IgG antibodies are dispensed into the wells. During a second incubation this anti IgG conjugate binds specifically to IgG antibodies resulting in the formation of enzyme-linked immune complexes. After a second washing step to remove unbound conjugate the immune complexes formed (in case of positive results) are detected by incubation with TMB substrate and development of a blue color. The blue color turns into yellow by stopping the enzymatic indicator reaction with sulfuric acid. The intensity of this color is directly proportional to the amount of Fasciola-specific IgG antibody in the sample specimen. Absorbance at 450 nm is read using an ELISA microtiter plate reader.