Human p53 ELISA Kit, 1 x 48-well (pre-coated)

CAT#: EA101181

Human p53 ELISA Kit, 1 x 48-well (pre-coated)

Product Images

Representative Standard Curve

Specifications

Product Data

• Format: 1 x 48-well (pre-coated)
• Assay Type: Solid Phase Sandwich ELISA
• Assay Length: 3h45min
• Signal: Colorimetric
• Curve Range: 3.1-100 U/ml
• Sample Type: Serum, Plasma, Cell lysate
• Sample Volume: 100 µl
• Specificity: Recognizes both natural and recombinant soluble human p53
• Sensitivity: 1.5 U/ml
• Cross Reactivity: No cross reactivity with other human soluble molecules
• Interference: No significant interference observed with available related molecules.
• Components: ELISA Kits include pre-coated strip plates, biotinylated secondary antibody, standards, controls (where applicable), buffers, streptavidin-HRP, TMB, stop reagents, and a detailed procedure.
• Background: This gene encodes a tumor suppressor protein containing transcriptional activation, DNA binding, and oligomerization domains. The encoded protein responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. Mutations in this gene are associated with a variety of human cancers, including hereditary cancers such as Li-Fraumeni syndrome. Alternative splicing of this gene and the use of alternate promoters result in multiple transcript variants and isoforms. Additional isoforms have also been shown to result from the use of alternate translation initiation codons (PMIDs: 12032546, 20937277). [provided by RefSeq, Feb 2013]
• Gene Symbol: TP53
• Standard Curve:
  
  Representative Standard Curve