Mouse Tdgf1 ELISA KIT (1 x 96 wells)

CAT#: EA102287

For quantitative detection of mouse Cripto in cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).

Specifications

Product Data

• Format: 8x12 divisible strips
• Assay Type: Sandwich ELISA kit of Quantitative Detection for mouse Cripto
• Assay Length: 3.5 hours incubations; 1 hour washing and analyzing samples
• Signal: Colorimetric
• Curve Range: 62.5pg/ml-4000pg/ml
• Specificity: This kit is used for quantitative detection of mouse Cripto
• Sensitivity: <10pg/ml
• Reactivities: Mouse
• Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
• Components:
  • 96-well plate precoated with anti- mouse Cripto antibody | 1
  • Lyophilized recombinant mouse Cripto standard | 10ng/tube×2
  • Biotinylated anti- mouse Cripto antibody | 130μl(dilution 1:100)
  • Avidin-Biotin-Peroxidase Complex (ABC) | 130μl(dilution 1:100)
  • Sample diluent buffer | 30ml
  • Antibody diluent buffer | 12ml
  • AR1106-3 ABC diluent buffer 12ml
  • AR1104 TMB color developing agent 10ml
  • AR1105 TMB stop solution 10ml
  • Adhesive cover | 4
• Background: Teratocarcinoma-derived growth factor 1 is a protein that in humans is encoded by the TDGF1 gene. It is mapped to 3p23-p21. The protein is an extracellular, membrane-bound signaling protein that plays an essential role in embryonic developmentand tumor growth. Mutations in this gene are associated with forebrain defects. Pseudogenes of this gene are found on chromosomes 2, 3, 6, 8, 19 and X. Alternate splicing results in multiple transcript variants.
• Gene Symbol: Tdgf1
• Standard Curve:
  
  Mouse Cripto/TDGF1 ELISA Kit standard curve