Rat Cdh1 ELISA KIT (1 x 96 wells)

CAT#: EA102310

For quantitative detection of rat E-Cadherin in cell culture supernates, serum and plasma(heparin).

Specifications

Product Data

• Format: 8x12 divisible strips
• Assay Type: Sandwich ELISA kit of Quantitative Detection for rat E-Cadherin
• Assay Length: 3.5 hours incubations; 1 hour washing and analyzing samples
• Signal: Colorimetric
• Curve Range: 156pg/ml-10000pg/ml
• Specificity: This kit is used for quantitative detection of rat E-Cadherin
• Sensitivity: <15pg/ml
• Reactivities: Rat
• Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
• Components:
  • 96-well plate precoated with anti- rat E-Cadherin antibody | 1
  • Lyophilized rat E-Cadherin standard | 10ng/tube×2
  • Biotinylated anti- rat E-Cadherin antibody | 130μl(dilution 1:100)
  • Avidin-Biotin-Peroxidase Complex (ABC) | 130μl(dilution 1:100)
  • Sample diluent buffer | 30ml
  • Antibody diluent buffer | 12ml
  • ABC diluent buffer | 12ml
  • TMB color developing agent | 10ml
  • TMB stop solution | 10ml
  • Adhesive cover | 4
• Background: Cadherin-1, also known as CAM 120/80 or epithelial cadherin (E-cadherin) or uvomorulin, is a protein that in humans is encoded by the CDH1 gene. E-cadherin is a Ca(2+)-dependent epithelial cell-cell adhesion molecule. Downregulation of E-cadherin expression often correlates with strong invasive potential and poor prognosis of human carcinomas. The gene spans a region of approximately 100 kb, and its location on chromosome 16q22.1. It contains 16 exons and a 65-kb-long intron 2. E-cadherin gene mutations may contribute to the development of diffusely growing gastric carcinomas. It plays a central part in the process of epithelial morphogenesis and acts as a strong invasion suppressor in experimental tumor cell systems. The standard product used in this kit is recombinant gene expression with the molecular mass of 120KDa.
• Gene Symbol: Cdh1
• Standard Curve:
  
  Rat E-Cadherin ELISA Kit standard curve