Human IL32 ELISA KIT (1 x 96 wells)

CAT#: EA102431

For quantitative detection of human IL-32 in cell culture supernates, serum and plasma (heparin, EDTA).

Specifications

Product Data

• Format: 8x12 divisible strips
• Assay Type: Sandwich ELISA kit of Quantitative Detection for Human IL-32
• Assay Length: 3.5 hours incubations; 1 hour washing and analyzing samples
• Signal: Colorimetric
• Curve Range: 62.5pg/ml-4000pg/ml
• Specificity: This kit is used for quantitative detection of Human IL-32
• Sensitivity: <10pg/ml
• Reactivities: Human
• Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
• Components:
  • 96-well plate precoated with anti- human IL-32 antibody | 1
  • Lyophilized recombinant human IL-32 standard | 10ng/tube×2
  • Biotinylated anti- human IL-32 antibody | 130μl(dilution 1:100)
  • Avidin-Biotin-Peroxidase Complex (ABC) | 130μl(dilution 1:100)
  • Sample diluent buffer | 30 ml
  • Antibody diluent buffer | 12ml
  • ABC diluent buffer | 12ml
  • TMB color developing agent | 10ml
  • TMB stop solution | 10ml
  • Adhesive cover | 4
• Background: Interleukin 32 (Il32) is a protein that in humans is encoded by the IL32 gene. This gene encodes a member of the cytokine family. The protein contains a tyrosine sulfation site, 3 potential N-myristoylation sites, multiple putative phosphorylation sites, and an RGD cell-attachment sequence. Expression of this protein is increased after the activation of T-cells by mitogens or the activation of NK cells by IL-2. This protein induces the production of TNFalpha from macrophage cells. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.
• Gene Symbol: IL32
• Standard Curve:
  
  Human IL-32 ELISA Kit Standard Curve