Human beta 2 Microglobulin (B2M) ELISA KIT (1 x 96 wells)

CAT#: EA102493

For quantitative detection of human B2M in cell culture supernates, serum, plasma (heparin, EDTA), saliva, urine and human milk.

Specifications

Product Data

• Format: 8x12 divisible strips
• Assay Type: Sandwich ELISA kit of Quantitative Detection for Human B2M
• Assay Length: 3.5 hours incubations; 1 hour washing and analyzing samples
• Signal: Colorimetric
• Curve Range: 156pg/ml-10000pg/ml
• Specificity: This kit is used for quantitative detection of Human B2M
• Sensitivity: <10pg/ml
• Reactivities: Human
• Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
• Components:
  • 96-well plate precoated with anti- Human B2M antibody | 1
  • Lyophilized recombinant Human B2M standard | 10ng/tubex2
  • Biotinylated anti- Human B2M antibody | 130ul(dilution 1:100)
  • Avidin-Biotin-Peroxidase Complex(ABC) | 130ul(dilution 1:100)
  • Sample diluent buffer | 30ml
  • Antibody diluent buffer | 12ml
  • ABC diluent buffer | 12ml
  • TMB color developing agent | 10ml
  • TMB stop solution | 10ml
  • Adhesive cover | 4
• Background: Beta-2 microglobulin also known as B2M is a component of MHC class I molecules, which are present on all nucleated cells (excludes red blood cells). In humans, the beta-2-microglobulin protein is encoded by the B2M gene. The protein has a predominantly beta-pleated sheet structure that can form amyloid fibrils in some pathological conditions. The encoded antimicrobial protein displays antibacterial activity in amniotic fluid. A mutation in this gene has been shown to result in hypercatabolic hypoproteinemia.
• Gene Symbol: B2M
• Standard Curve:
  
  Human B2M ELISA Kit standard curve