Rat Sema3f ELISA KIT (1 x 96 wells)

CAT#: EA102515

For quantitative detection of rat SEMA3F in cell culture supernates, serum and plasma (heparin, EDTA, citrate).

Specifications

Product Data

• Format: 8x12 divisible strips
• Assay Type: Sandwich ELISA kit of Quantitative Detection for Rat SEMA3F
• Assay Length: 3.5 hours incubations; 1 hour washing and analyzing samples
• Signal: Colorimetric
• Curve Range: 31.2pg/ml-2000pg/ml
• Specificity: This kit is used for quantitative detection of Rat SEMA3F
• Sensitivity: <10pg/ml
• Reactivities: Rat
• Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
• Components:
  • 96-well plate precoated with anti- rat SEMA3F antibody | 1
  • Lyophilized recombinant rat SEMA3F standard | 10ng/tube×2
  • Biotinylated anti- rat SEMA3F antibody | 130μl(dilution 1:100)
  • Avidin-Biotin-Peroxidase Complex (ABC) | 130μl(dilution 1:100)
  • Sample diluent buffer | 30 ml
  • Antibody diluent buffer | 12ml
  • ABC diluent buffer | 12ml
  • TMB color developing agent | 10ml
  • TMB stop solution | 10ml
  • Adhesive cover | 4
• Background: Semaphorin-3F is a protein that in humans is encoded by the SEMA3F gene. It is mapped to 8q32. The semaphorins are a family of proteins that are involved in signaling. All the family members have a secretion signal, a 500-amino acid sema domain, and 16 conserved cysteine residues. Sequence comparisons have grouped the secreted semaphorins into 3 general classes, all of which also have an immunoglobulin domain. The semaphorin 3 family, consisting of human semaphorins 3A-G, chicken collapsin, and mouse semaphorins 3A-G, all have a basic domain at the C terminus. Chicken collapsin contributes to path finding by axons during development by inhibiting extension of growth cones through an interaction with a collapsin response mediator protein of relative molecular mass 62K , a putative homolog of an axonal guidance associated UNC33 gene product. SEMA3F is a secreted member of the semaphorin III family.
• Gene Symbol: Sema3f
• Standard Curve:
  
  Rat SEMA3F ELISA Kit Standard Curve