Rat Eda ELISA KIT (1 x 96 wells)

CAT#: EA102582

For quantitative detection of rat EDA in cell culture supernates, serum and plasma (heparin, EDTA).

Specifications

Product Data

• Format: 8x12 divisible strips
• Assay Type: Sandwich ELISA kit of Quantitative Detection for Rat EDA
• Assay Length: 3.5 hours incubations; 1 hour washing and analyzing samples
• Signal: Colorimetric
• Curve Range: 93.7pg/ml-6000pg/ml
• Specificity: This kit is used for quantitative detection of Rat EDA
• Sensitivity: <50pg/ml
• Reactivities: Rat
• Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
• Components:
  • 96-well plate precoated with anti- rat EDA antibody | 1
  • Lyophilized recombinant rat EDA standard | 10ng/tube×2
  • Biotinylated anti- rat EDA antibody | 130μl(dilution 1:100)
  • Avidin-Biotin-Peroxidase Complex (ABC) | 130μl(dilution 1:100)
  • Sample diluent buffer | 30ml
  • Antibody diluent buffer | 12ml
  • ABC diluent buffer | 12ml
  • TMB color developing agent | 10ml
  • TMB stop solution | 10ml
  • Adhesive cover | 4
• Background: Ectodysplasin A (EDA) is a protein that in humans is encoded by the EDA gene. It is mapped to Xq22. The protein encoded by this gene is a type II membrane protein that can be cleaved by furin to produce a secreted form. The encoded protein, which belongs to the tumor necrosis factor family, acts as a homotrimer and may be involved in cell-cell signaling during the development of ectodermal organs. Defects in this gene are a cause of ectodermal dysplasia, anhidrotic, which is also known as X-linked hypohidrotic ectodermal dysplasia. Several transcript variants encoding many different isoforms have been found for this gene.
• Gene Symbol: Eda
• Standard Curve:
  
  Rat EDA ELISA Kit Standard Curve