Rat Cdh5 ELISA KIT (1 x 96 wells)

CAT#: EA102611

For quantitative detection of rat VE-Cadherin/CD144 in cell culture supernates, serum and plasma (heparin, EDTA).

Specifications

Product Data

• Format: 8x12 divisible strips
• Assay Type: Sandwich ELISA kit of Quantitative Detection for Rat VE-Cadherin/CD144
• Assay Length: 3.5 hours incubations; 1 hour washing and analyzing samples
• Signal: Colorimetric
• Curve Range: 312pg/ml-20000pg/ml
• Specificity: This kit is used for quantitative detection of Rat VE-Cadherin/CD144
• Sensitivity: <50pg/ml
• Reactivities: Rat
• Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
• Components:
  • 96-well plate precoated with anti- rat VE-Cadherin/CD144 antibody | 1
  • Lyophilized recombinant rat VE-Cadherin/CD144 standard | 20ng/tube×2
  • Biotinylated anti- rat VE-Cadherin/CD144 antibody | 130μl(dilution 1:100)
  • Avidin-Biotin-Peroxidase Complex (ABC) | 130μl(dilution 1:100)
  • Sample diluent buffer | 30 ml
  • Antibody diluent buffer | 12ml
  • ABC diluent buffer | 12ml
  • TMB color developing agent | 10ml
  • TMB stop solution | 10ml
  • Adhesive cover | 4
• Background: Cadherin 5, type 2 or VE-cadherin also known as CD144, is a type of cadherin. It is encoded by the human gene CDH5. This gene is mapped to 19p14. VE-cadherin is a classical cadherin from the cadherin superfamily and the gene is located in a six-cadherin cluster in a region on the long arm of chromosome 16 that is involved in loss of heterozygosity events in breast and prostate cancer. The encoded protein is a calcium-dependent cell–cell adhesion glycoprotein composed of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Functioning as a classic cadherin by imparting to cells the ability to adhere in a homophilic manner, the protein may play an important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions.
• Gene Symbol: Cdh5
• Standard Curve:
  
  Rat VE-Cadherin/CD144 ELISA Kitstandard curve