Human Protein C (PROC) ELISA KIT (1 x 96 wells)

CAT#: EA102657

For quantitative detection of human PROC/Protein C in cell culture supernates, serum and plasma (heparin, EDTA) and urine.

Specifications

Product Data

• Format: 8x12 divisible strips
• Assay Type: Sandwich ELISA kit of Quantitative Detection for Human PROC/Protein C
• Assay Length: 3.5 hours incubations; 1 hour washing and analyzing samples
• Signal: Colorimetric
• Curve Range: 0.78ng/ml-50ng/ml
• Specificity: This kit is used for quantitative detection of Human PROC/Protein C
• Sensitivity: <0.1ng/ml
• Reactivities: Human
• Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
• Components:
  • 96-well plate precoated with anti- human PROC/Protein C antibody | 1
  • Lyophilized recombinant human PROC/Protein C standard | 50ng/tube×2
  • Biotinylated anti- human PROC/Protein C antibody | 130μl(dilution 1:100)
  • Avidin-Biotin-Peroxidase Complex (ABC) | 130μl(dilution 1:100)
  • Sample diluent buffer | 30 ml
  • Antibody diluent buffer | 12ml
  • ABC diluent buffer | 12ml
  • TMB color developing agent | 10ml
  • TMB stop solution | 10ml
  • Adhesive cover | 4
• Background: Protein C(PROC), also called PC, is a zymogenic (inactive) protein, the activated form of which plays an important role in regulating blood clotting, inflammation, cell death and maintaining the permeability of blood vessel walls in humans and other animals. The PROC gene is mapped to 2q14.3. This gene encodes a vitamin K-dependent plasma glycoprotein. The encoded protein is cleaved to its activated form by the thrombinthrombomodulin complex. This activated form contains a serine protease domain and functions in degradation of the activated forms of coagulation factors V and VIII. Mutations in this gene have been associated with thrombophilia due to protein C deficiency, neonatal purpura fulminans, and recurrent venous thrombosis.
• Gene Symbol: PROC
• Standard Curve:
  
  Human PROC/Protein C ELISA Kitstandard curve