Mouse Chil3 ELISA KIT (1 x 96 wells)

CAT#: EA102687

For quantitative detection of mouse CHI3L3 in cell culture supernates, serum and plasma (heparin, EDTA, citrate).

Specifications

Product Data

• Format: 8x12 divisible strips
• Assay Type: Sandwich ELISA kit of Quantitative Detection for Mouse CHI3L3
• Assay Length: 3.5 hours incubations; 1 hour washing and analyzing samples
• Signal: Colorimetric
• Curve Range: 312pg/ml-20000pg/ml
• Specificity: This kit is used for quantitative detection of Mouse CHI3L3
• Sensitivity: <50pg/ml
• Reactivities: Mouse
• Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
• Components:
  • 96-well plate precoated with anti- mouse CHI3L3 antibody | 1
  • Lyophilized recombinant mouse CHI3L3 standard | 20ng/tube×2
  • Biotinylated anti- mouse CHI3L3 antibody | 130μl(dilution 1:100)
  • Avidin-Biotin-Peroxidase Complex (ABC) | 130μl(dilution 1:100)
  • Sample diluent buffer | 30ml
  • Antibody diluent buffer | 12ml
  • ABC diluent buffer | 12ml
  • TMB color developing agent | 10ml
  • TMB stop solution | 10ml
  • Adhesive cover | 4
• Background: Chitinase 3-like 3, also known as ECF-L and Ym1, is a secreted 377 amino acid lectin that binds heparin and GlcN oligomers. This gene is mapped to 3 F2.2; 3 46.49 Cm. CHI3L3 is produced primarily by macrophages during inflammation. ECF-L shares homology with chitinase family proteins, but lacks enzymatic activity.
• Gene Symbol: Chil3
• Standard Curve:
  
  Mouse CHI3L3 ELISA Kit Standard Curve