Aga (NM_001005847) Mouse Untagged Clone

CAT#: MC208116

Aga (untagged) - Mouse aspartylglucosaminidase (Aga), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Aga
• Synonyms: AW060726
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC208116 representing NM_001005847
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCGGAAGTCGAATCTGTCTCTGCTTCTCCTACTGCTGGTCCTGGGCATGCCCCTGGTGCGGGGCT
  CCAGCCCTCTGCCCCTGGTCGTCAACACTTGGCCTTTTAAGAATGCCACTGAAGCAGCGTGGTGGACATT
  GCTATCTGGAGGTTCTGCCCTGGATGCAGTGGAGAACGGCTGTGCTGTGTGTGAGAAGGAGCAGTGTGAT
  GGGACTGTAGGCTTTGGAGGAAGTCCTGATGAAGGTGGCGAAACCACCCTGGATGCCATGATAATGGATG
  GCACTGCCATGGATGTGGGAGCAGTGGGAGGCCTTAGAAGAATTAAAAACGCGATTGGCGTGGCGCGGAG
  AGTCCTGGAGCATACCACACACACGCTTTTAGTGGGGGACTCAGCCACCAAGTTTGCTGAAAGTATGGGG
  TTTACTAATGAGGACTTGTCTACCAAAACCTCAAGAGATCTTCATTCAGATTGGCTTTCTCGAAATTGCC
  AGCCAAATTATTGGAGAAATGTTATTCCAGATCCCTCAAAATACTGTGGACCCTACAAACCATCTGGTTT
  CTTAAAGCAGAGTATTTCTCCCCACAAAGAAGAAGTGGATATCCACAGCCATGATACTATTGGCATGGTT
  GTAATCCATAAGACGGGACATACTGCTGCTGGCACATCCACAAATGGTATAAAATTCAAAATACCTGGTC
  GTGTAGGGGATTCACCAATCCCTGGAGCCGGAGCCTATGCTGATGACACGGCTGGAGCAGCTGCAGCCAC
  TGGCGATGGTGACACACTCCTGCGCTTTCTGCCGAGCTACCAAGCTGTAGAATATATGAGAGGAGGAGAT
  GACCCAGCCATAGCTTGCCAAAAAGTGATTTTAAGAATTCAGAAATACTATCCAAACTTCTTTGGAGCGG
  TCATATGTGCCAGTGTGAACGGAAGTTATGGTGCTGCTTGCAACAAACTTCCAACATTTACACAATTTAG
  TTTCATGGTTTCTAATTCTTTACACAATGAGCCAACCGAAAAAAAAGTAGACTGCATCTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001005847
• Insert Size: 1041 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001005847.2, NP_001005847.1
• RefSeq Size: 1307 bp
• RefSeq ORF: 1041 bp
• Locus ID: 11593
• Cytogenetics: 8 B1.3
• Gene Summary: This gene encodes an amidase enzyme that participates in the breakdown of glycoproteins in the cell. The encoded protein undergoes proteolytic processing to generate a mature enzyme. Mice lacking the encoded protein exhibit accumulation of aspartylglucosamine along with lysosomal vacuolization, axonal swelling in the gracile nucleus and impaired neuromotor coordination. Alternative splicing results in multiple transcript variants encoding different isoforms that may undergo similar processing to generate mature protein. [provided by RefSeq, Oct 2015]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.