Tnnt2 (NM_001130175) Mouse Untagged Clone

CAT#: MC209562

Tnnt2 (untagged) - Mouse troponin T2, cardiac (Tnnt2), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Tnnt2
• Synonyms: cTnT; Tnt
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC209562 representing NM_001130175
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCCTGCCCTCAGCCAGTCCCTGCTGAGGCTGAACAGATACCTCAAGACCTGTGTGCAGTCCCTGTTC
  AGAGAGCCGAGAGCATGTCTGACGCCGAGGAGGTGGTGGAGGAGTACGAGGAGGAACAGGAAGAAGCTGT
  GGAAGAGGAAGACTGGAGTGAAGAAGAGGAGGACGAGCAAGAGGAGGCAGTGGAGGAGGAGGAGGCTGGT
  GGGGCTGAACCTGAGCCTGAGGGTGAGGCTGAGACAGAGGAGGCCAACGTAGAAGAGGTTGGTCCTGATG
  AAGAAGCCAAAGATGCTGAAGAAGGTCCAGTAGAGGACACCAAACCCAAGCCCAGCAGGCTCTTCATGCC
  CAACTTGGTGCCACCCAAGATCCCCGATGGAGAGAGAGTGGACTTTGATGACATCCACAGGAAGCGCGTG
  GAGAAGGACCTGAATGAGCTACAGACTCTGATCGAGGCTCACTTCGAGAACAGGAAGAAGGAGGAAGAGG
  AGCTGATTTCCCTCAAAGACAGGATCGAAAAGCGTCGGGCAGAGCGGGCCGAGCAGCAGCGTATTCGCAA
  TGAGCGGGAGAAGGAAAGGCAGAACCGCCTGGCTGAAGAGAGGGCCCGGCGTGAGGAGGAGGAGAACAGG
  AGGAAGGCTGAGGATGAGGCCCGGAAGAAGAAGGCTCTGTCCAACATGATGCACTTTGGAGGGTACATCC
  AGAAGACAGAGCGGAAGAGTGGGAAGAGACAGACAGAGAGAGAGAAGAAGAAGAAGATCCTGGCAGAGAG
  GAGGAAGGCGCTGGCCATCGACCACCTGAATGAAGACCAACTGAGAGAGAAGGCCAAGGAGCTGTGGCAG
  AGTATTCACAACCTGGAGGCTGAGAAGTTCGACCTGCAGGAAAAGTTCAAGCAGCAGAAATACGAAATCA
  ACGTTCTGCGAAACCGGATCAATGACAACCAGAAAGTCTCCAAAACTCGTGGGAAGGCCAAAGTCACCGG
  GCGTTGGAAATAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001130175
• ORF Size: 993 bp
• Insert Size: 993
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info

Reference Data

• RefSeq: NM_001130175.1, NP_001123647.1
• RefSeq Size: 1228
• RefSeq ORF: 993
• Locus ID: 21956
• Gene Summary: Troponin T is the tropomyosin-binding subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity. [UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) uses an alternate splice site in the 5' region, and initiates translation at an alternate start codon, compared to variant 1. The encoded isoform (g) has a distinct N-terminus and is longer than isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.