Tnfsfm13 (NM_001034097) Mouse Untagged Clone

CAT#: MC215179

BC096441 (untagged) - Mouse cDNA sequence BC096441 (BC096441), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Tnfsfm13
• Synonyms: Tnfsf12-Tnfsf13; Tnfsf12Tnfsf13; Tnfsf12tnfsf13; Twe-pril
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC215179 representing NM_001034097
  Red=Cloning site Blue=ORF
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCGCCCGTCGGAGCCAGAGGCGGAGGGGGCGCCGGGGGGAGCCGGGCACCGCCCTGCTGGCCCCGC
  TGGTGCTGAGCCTGGGCCTGGCGCTGGCCTGCCTTGGCCTCCTGCTGGTCGTGGTCAGCCTGGGGAGCTG
  GGCAACGCTGTCTGCCCAGGAGCCTTCTCAGGAGGAGCTGACAGCAGAGGACCGCCGGGAGCCCCCTGAA
  CTGAATCCCCAGACAGAGGAAAGCCAGGATGTGGTACCTTTCTTGGAACAACTAGTCCGGCCTCGAAGAA
  GTGCTCCTAAAGGCCGGAAGGCGCGGCCTCGCCGAGCTATTGCAGCCCATTATGAGGTTCATCCTCGGCC
  AGGACAGGATGGAGCACAAGCAGGTGTGGATGGGACAGTGAGTGGCTGGGAAGAGACCAAAATCAACAGC
  TCCAGCCCTCTGCGCTACGACCGCCAGATTGGGGAATTTACAGTCATCAGGGCTGGGCTCTACTACCTGT
  ACTGTCAGGTGCACTTTGATGAGGGAAAGGCTGTCTACCTGAAGCTGGACTTGCTGGTGAACGGTGTGCT
  GGCCCTGCGCTGCCTGGAAGAATTCTCAGCCACAGCAGCAAGCTCTCCTGGGCCCCAGCTCCGTTTGTGC
  CAGACAGAGCTGCAAAGCCTAAGGCGGGAGGTGAGCCGGCTGCAGCGGAGTGGAGGGCCTTCCCAGAAGC
  AGGGAGAGCGCCCATGGCAGAGCCTCTGGGAGCAGAGTCCTGATGTCCTGGAAGCCTGGAAGGATGGGGC
  GAAATCTCGGAGAAGGAGAGCAGTACTCACCCAGAAGCACAAGAAGAAGCACTCAGTCCTGCATCTTGTT
  CCAGTTAACATTACCTCCAAGGACTCTGACGTGACAGAGGTGATGTGGCAACCAGTACTTAGGCGTGGGA
  GAGGCCTGGAGGCCCAGGGAGACATTGTACGAGTCTGGGACACTGGAATTTATCTGCTCTATAGTCAGGT
  CCTGTTTCATGATGTGACTTTCACAATGGGTCAGGTGGTATCTCGGGAAGGACAAGGGAGAAGAGAAACT
  CTATTCCGATGTATCAGAAGTATGCCTTCTGATCCTGACCGTGCCTACAATAGCTGCTACAGTGCAGGTG
  TCTTTCATTTACATCAAGGGGATATTATCACTGTCAAAATTCCACGGGCAAACGCAAAACTTAGCCTTTC
  TCCGCATGGAACATTCCTGGGGTTTGTGAAACTATGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001034097
• Insert Size: 1227 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC096441, AAH96441
• RefSeq Size: 1511 bp
• RefSeq ORF: 1233 bp
• Locus ID: 619441
• Cytogenetics: 11 B3
• Gene Summary: This gene represents read-through transcription between neighboring genes encoding tumor necrosis factor family members. This results a hybrid protein composed of the cytoplasmic and transmembrane domains of family member 12 fused to the C-terminal domain of family member 13. The homologous fusion protein in human is known to be membrane-anchored and presents the receptor-binding domain of family member 13 at the cell surface. It stimulates cycling in T- and B-lymphoma cell lines. Alternative splicing of this mouse gene results in multiple transcript variants. [provided by RefSeq, Oct 2010]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (a).