Pus1 (NM_001025561) Mouse Untagged Clone

CAT#: MC215822

Pus1 (untagged) - Mouse pseudouridine synthase 1 (Pus1), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Pus1
• Synonyms: A730013B20Rik; MPUS1; mPus1p
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC215822 representing NM_001025561
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCTTCCCCCGCCTGTGGGCCGCACTGCTGAGAAACTGGGGACGGTGGACGGCGCGCCCAGGCCCGC
  GCGTGCCCGGCCTGCCGCCTATGGCTGGAAACAAGGTGCCACCAGCGCTGGCCTCACACCAACCGGACAG
  GAAGGGCCGAGGTGGCTGGGTCTGGGAGGAGACGGAGCATCCTGCAAAGAGGGTCAAGGGCGGCGAGGAT
  GAAGAGCCCCCACGCAAGCTGCCAAAGCGAAAAATTGTGCTGCTCATGGCCTACTCGGGCAAGGGCTACC
  ACGGCATGCAGAGGAATCTGGGGTCCTCCCAGTTCAGGACCATCGAAGATGACTTGGTATCGGCCTTAGT
  CCAGGCAGGTTGTATCCCTGAAAACCATGGCACAGATATGAGGAAGATGTCCTTCCAGCGGTGTGCTCGA
  ACAGACAAGCCTTTGAGGACCTCTGCCTGCTTTTGCCTGTACACTGGCTTCCTCTTCCACTGGGGTGTGT
  CTGCAGCAGGGCAGGTGGTATCCCTAAAGGTGTGGCTGATTGATGACATTCTGGACAAGATCAACAGCCA
  CCTTCCATCCCACATTCGGATTCTGGGATTGAAGAGGGTCACAGGCGGGTTCAACTCCAAGAACAAGTGT
  GACGCCAGGACCTACTGCTACATGCTGCCCACCTTTGCCTTTGCTCACAAGGACCGGGATGTACAGGACG
  AGAGCTATCGCCTGAGTGCAGAGACCCTGCAGCAGGTCAACCGCCTTCTGGCCTGCTACAAAGGCACCCA
  TAACTTCCATAACTTCACCTCGCAGAAGGGCCCAAGAGAGCCCAGTGCGCGCCGCTACATTCTGGAGATG
  TACTGCGAAGAGCCCTTCGTACGTGAGGGCCTGGAGTTTGCTGTGATCAAGGTGAAGGGCCAGAGCTTCA
  TGATGCACCAGATCCGGAAGATGGTTGGCCTGGTGGTAGCCATTGTGAAGGGTTATGCCCCTGAGAGTGT
  CCTGGAGCGCAGCTGGGGAGAGGAGAAGGTGGATGTGCCCAAGGCACCCGGGCTTGGCTTAGTCCTGGAG
  AGGGTTCACTTTGAAAAGTACAATCAACGCTTTGGCGGTGACGGGCTGCATGAGCCCCTGGACTGGACGC
  AGGAGGAAGGGAAGGTGACTGCTTTCAAGGAGCAGTACATTTACCCTACCATTGTCAGCACTGAGCGGGA
  TGAGCGGTCCATGGCCCAGTGGCTCAATACTTTGCCGATCCACAACTTCAGTGGCACTGCACTTGGAGCG
  GCTGACACAGGTGCCAAGGTCCCCAGTTCCCTGGAGGGCAGTGAAGGGGATGGAGACACTGACTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001025561
• Insert Size: 1326 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001025561.3, NP_001020732.1
• RefSeq Size: 1868 bp
• RefSeq ORF: 1326 bp
• Locus ID: 56361
• Cytogenetics: 5 F