Tyms (BC020139) Mouse Untagged Clone

CAT#: MC218262

Tyms (untagged) - Mouse thymidylate synthase (cDNA clone MGC:28246 IMAGE:3994204), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Tyms
• Synonyms: TS
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC020139
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGGTGGTTGGCTCCGAGCTGCAGTCCGATGCTCAGCAGCTGAGCGCGGAAGCCCCGCAGCATGGAG
  AACTCCAGTACCTGAGGCAGGTGGAACACATTTTGCGCTGCGGCTTCAAGAAGGAGGACCGCACGGGCAC
  AGGCACCCTGTCGGTGTTCGGCATGCAGGCACGATACAGCCTGAGAGATGAATTTCCTCTGCTCACAACC
  AAACGAGTGTTCTGGAAGGGTGTTTTGGAGGAGTTGTTGTGGTTTATCAAGGGATCCACAAATGCTAAAG
  AATTGTCCTCCAAGGGAGTGAGAATCTGGGATGCCAATGGATCCCGAGATTTTCTGGACAGCTTGGGATT
  TTCTGCCCGACAGGAAGGGGACCTGGGCCCAGTTTATGGTTTCCAATGGAGGCATTTTGGAGCAGAGTAC
  AAAGATATGGATTCAGATTACTCGGGACAAGGAGTAGACCAGCTGCAAAAAGTGATTGACACCATCAAAA
  CCAACCCTGATGACAGAAGAATCATCATGTGTGCCTGGAACCCAAAAGATCTTCCCCTGATGGCACTGCC
  TCCTTGCCATGCCCTCTGTCAGTTCTATGTGGTGAATGGGGAACTGTCTTGCCAGCTTTACCAGAGGTCA
  GGAGATATGGGTCTGGGCGTGCCCTTCAACATTGCCAGCTATGCTCTGCTCACCTACATGATTGCACATA
  TCACAGGCCTGCAGCCAGGTGATTTTGTCCACACTTTGGGAGATGCACATATTTACCTGAATCATATAGA
  GCCGCTGAAAATTCAGCTACAGCGAGAACCAAGACCTTTCCCAAAGCTCAAAATCCTTCGAAAAGTTGAG
  ACAATCGATGATTTCAAAGTTGAAGACTTTCAGATTGAAGGGTATAATCCACATCCAACGATTAAAATGG
  AAATGGCTGTTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: BC020139
• ORF Size: 924 bp
• Insert Size: 924
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).

Reference Data

• RefSeq: BC020139, AAH20139
• RefSeq Size: 986
• RefSeq ORF: 923
• Locus ID: 22171
• Gene Summary: This gene encodes an enzyme that catalyzes the methylation of deoxyuridylate to deoxythymidylate using 5,10-methylenetetrahydrofolate as a cofactor. This function maintains the thymidine-5-prime monophosphate concentration critical for DNA replication and repair. The encoded enzyme is a target for cancer chemotherapeutic agents. The majority of transcripts for this gene lack a 3' UTR (PMID: 3022294, 3444407). The stop codon in these transcripts is UAA, compared to the UAG found in the genome and longer transcripts, as the polyA site is located within the stop codon (PMID: 3444407, 2157203). A related pseudogene has been identified on chromosome 10. [provided by RefSeq, Mar 2010]