Psap (NM_001146124) Mouse Untagged Clone

CAT#: MC218817

Psap (untagged) - Mouse prosaposin (Psap), transcript variant 6, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Psap
• Synonyms: AI037048; SGP-1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC218817 representing NM_001146124
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGGGCTCCGGGCAAACTCTCGTGATGCCTCTGACCAGCCCTGTCCAAGACCCGAAGACATGCTCTG
  GGGGCTCAGCAGTGCTGTGCAGAGATGTGAAGACGGCGGTGGACTGTGGGGCCGTGAAGCACTGCCAGCA
  GATGGTCTGGAGCAAGCCCACAGCGAAATCCCTTCCTTGCGACATATGCAAAACTGTTGTCACCGAAGCT
  GGGAACTTGCTGAAAGATAATGCTACGCAGGAGGAGATCCTTCATTACCTGGAGAAGACCTGTGAGTGGA
  TTCATGACTCCAGCCTGTCGGCCTCGTGCAAGGAGGTGGTTGACTCTTACCTGCCTGTCATCCTGGACAT
  GATTAAGGGCGAGATGAGCAACCCTGGGGAAGTGTGCTCTGCGCTCAACCTCTGCCAGTCCCTTCAGGAG
  TACTTGGCCGAGCAAAACCAGAAACAGCTTGAGTCCAACAAGATCCCGGAGGTGGACATGGCCCGTGTGG
  TTGCCCCCTTCATGTCCAACATCCCTCTCCTGCTGTACCCTCAGGATCACCCCCGCAGCCAGCCCCAACC
  TAAGGCTAACGAGGACGTCTGCCAGGACTGTATGAAGCTGGTGTCTGATGTCCAGACTGCTGTGAAGACC
  AACTCCAGCTTTATCCAGGGCTTCGTGGACCACGTGAAGGAGGATTGTGACCGCTTGGGGCCAGGCGTGT
  CTGACATATGCAAGAACTACGTGGACCAGTATTCCGAGGTCTGTGTCCAGATGTTGATGCACATGCAACC
  CAAGGAAATCTGTGTGCTGGCTGGCTTCTGTAATGAGGTCAAGAGAGTGCCAATGAAGACTCTGGTCCCT
  GCCACCGAGACCATTAAGAACATCCTCCCTGCCCTGGAGATGATGGACCCCTATGAGCAGAATCTGGTCC
  AGGCCCACAATGTGATTTTATGCCAGACCTGTCAGTTTGTGATGAATAAGTTTTCTGAGCTGATTGTCAA
  TAATGCCACTGAGGAGCTCCTAGTTAAAGGTTTGAGCAACGCATGCGCACTGCTCCCCGATCCTGCCAGA
  ACCAAGTGCCAGGAGGTGGTGGGAACATTTGGCCCCTCCCTGTTGGACATCTTTATCCATGAGGTAAACC
  CCAGCTCTCTGTGCGGTGTGATCGGCCTCTGTGCTGCCCGCCCGGAGTTGGTGGAGGCACTTGAGCAGCC
  TGCGCCAGCCATTGTATCTGCACTGCTCAAAGAGCCCACACCGCCAAAGCAGCCCGCACAGCCCAAGCAG
  TCGGCATTGCCCGCCCATGTGCCTCCTCAGAAGAATGGTGGGTTCTGTGAGGTGTGCAAGAAACTGGTCC
  TCTATTTGGAACATAACCTGGAGAAAAACAGCACCAAGGAGGAAATCCTGGCCGCACTTGAGAAGGGCTG
  CAGCTTCCTGCCAGACCCTTACCAGAAGCAGTGCGATGACTTTGTGGCTGAGTATGAGCCCTTGCTATTG
  GAGATCCTCGTGGAAGTGATGGATCCTGGATTTGTGTGCTCGAAAATTGGAGTTTGCCCTTCTGCCTATA
  AGCTGCTGCTGGGAACCGAGAAGTGTGTCTGGGGCCCTAGCTACTGGTGTCAGAACATGGAGACTGCCGC
  CCGATGCAATGCTGTCGATCATTGCAAACGCCATGTGTGGAACTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001146124
• ORF Size: 1656 bp
• Insert Size: 1656
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).

Reference Data

• RefSeq: NM_001146124.1, NP_001139596.1
• RefSeq Size: 2583
• RefSeq ORF: 1656
• Locus ID: 19156
• Gene Summary: This gene encodes a multifunctional glycoprotein that plays a role in the intracellular metabolism of various sphingolipids or secreted into the plasma, milk or cerebrospinal fluid. The encoded protein undergoes proteolytic processing to generate four different polypeptides known as saposin A, B, C or D, that are required for the hydrolysis of certain sphingolipids by lysosomal hydrolases. Alternately, the encoded protein is secreted into body fluids where it exhibits neurotrophic and myelinotrophic activities. A complete lack of the encoded protein is fatal to mice either at the neonatal stage or within the first month due to severe leukodystrophy and sphingolipid accumulation. Alternative splicing results in multiple transcript variants encoding different isoforms that may undergo similar processing to generate the mature saposins. [provided by RefSeq, Sep 2015]
  Transcript Variant: This variant (6) uses an alternate exon for its 5' UTR and start codon and lacks an exon in the 3' coding region, compared to variant 2, resulting in a protein (isoform F) with a novel N-terminus and shorter C-terminus, compared to isoform B.