Adarb1 (NM_001024837) Mouse Untagged Clone

CAT#: MC220881

Adarb1 (untagged) - Mouse adenosine deaminase, RNA-specific, B1 (Adarb1), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Adarb1
• Synonyms: 1700057H01Rik; Adar2; AW124433; AW558573; BB220382; D10Bwg0447e; Red1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC220881 representing NM_001024837
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATATAGAAGATGAAGAGAATATGAGTTCCAGCAGCACTGATATTAAAGAAAACCGCAATCTGGACA
  ACATGCCCCCCAAGGACAGCAGCACACCTGGGCCTGGCGAGGGTATTCCGCTCTCCAACGGGGGTGGTGG
  TAGCACCAGCAGGAAGCGGCCCCTGGAGGAGGGCAGCAATGGTCACTCCAAGTACCGCCTGAAGAAGCGA
  AGGAAAACACCAGGGCCTGTTCTGCCCAAGAACGCCCTGATGCAGCTGAACGAGATCAAACCTGGCTTAC
  AGTACATGCTGCTGTCCCAGACAGGACCCGTGCATGCACCTCTGTTTGTCATGTCTGTGGAGGTAAACGG
  GCAGGTTTTTGAGGGCTCTGGCCCTACAAAGAAAAAGGCAAAACTCCATGCTGCTGAGAAGGCCCTGAGG
  TCTTTTGTCCAGTTTCCCAACGCCTCCGAGGCCCACCTAGCCATGGGAAGGACCCTCTCTGTGAACACAG
  ACTTCACGTCTGACCAGGCTGACTTCCCTGACACACTCTTCAATGGCTTCGAGACTCCAGACAAGTCAGA
  GCCACCCTTCTACGTAGGCTCCAACGGGGATGACTCGTTCAGCTCAAGTGGAGATGTCAGCCTATCGGCC
  TCCCCAGTGCCTGCCAGCCTTACCCAGCCTCCTCTGCCCATCCCACCACCATTCCCACCCCCAAGTGGGA
  AGAATCCCGTGATGATCTTGAATGAGCTACGCCCAGGGCTGAAGTATGACTTCCTCTCTGAGAGTGGGGA
  GAGCCACGCCAAGAGCTTCGTCATGTCCGTGGTGGTAGATGGCCAGTTCTTTGAGGGCTCAGGAAGAAAC
  AAGAAGCTTGCCAAGGCCCGGGCTGCACAGTCTGCCTTGGCTACTGTCTTCAATTTGCACTTGGACCAAA
  CACCATCTCGCCAGCCTGTCCTCAGTGAGGGTCTTCAGTTGCATTTGCCACAGGTATTGGCAGATGCTGT
  CTCCCGCCTGGTCCTGGGTAAGTTCAGTGACCTGACAGACAACTTTTCCTCCCCTCATGCACGAAGGAAA
  GTGCTCTCTGGAGTAGTGATGACCACAGGTACAGATGTCAAAGATGCCAAGGTGATAAGTGTTTCGACAG
  GGACGAAGTGTATCAACGGTGAATACATGAGTGACCGTGGCCTCGCACTCAATGACTGCCACGCAGAGAT
  AATCTCCCGAAGGTCCCTGCTCAGGTTTCTTTATGCACAGCTCGAGCTTTATTTAAATAACAAAGAAGAC
  CAGAAAAAGTCCATATTTCAGAAGTCAGAGCGGGGTGGGTTCCGGCTGAAGGATACCGTGCAGTTCCACC
  TGTACATCAGCACCTCGCCCTGCGGAGACGCCAGAATATTCTCTCCCCACGAGCCCGTGCTAGAGGGTAT
  GACGCCAGACTCTCACCAGCTGACAGAACCAGCAGATAGACATCCGAATCGCAAAGCAAGGGGACAGCTA
  CGGACGAAAATAGAGTCTGGCGAGGGGACAATCCCTGTGCGCTCAAATGCCAGCATCCAGACCTGGGACG
  GGGTGCTGCAGGGGGAACGGCTGCTCACCATGTCCTGCAGTGACAAGATAGCACGCTGGAACGTGGTGGG
  CATCCAGGGGTCCCTGCTCAGCATTTTCGTGGAACCCATCTACTTCTCCAGCATCATCTTGGGCAGCCTG
  TACCACGGGGACCACCTCTCCAGGGCCATGTACCAGCGGATCTCCAACATAGAGGACCTGCCACCGCTCT
  ACACTCTCAACAAGCCCCTGCTCAGCGGCATCAGCAATGCAGAGGCACGGCAGCCAGGGAAGGCACCCAA
  CTTCAGTGTCAACTGGACAGTGGGTGACGCCACCATTGAGGTCATCAATGCCACAACAGGGAAGGATGAG
  CTTGGCCGCCCATCCCGCCTGTGTAAGCACGCGCTGTACTGTCGCTGGATGCGTGTACACGGCAAGGTTC
  CCCCCCACCTGCTGCGCACCAAGATCACTAAGCCTACCACATACCACGAGTCCAAGCTGGCAGCGAGGGA
  GTACCAGGCTGCCAAGGCCCGTCTGTTCACTGCCTTCATCAAGGCGGGGCTGGGCGCCTGGGTGGAGAAG
  CCCACAGAGCAGGACCAGTTCTCCTTCACTCCCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001024837
• Insert Size: 2136 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001024837.2, NP_001020008.1
• RefSeq Size: 6602 bp
• RefSeq ORF: 2136 bp
• Locus ID: 110532
• Cytogenetics: 10 39.72 cM
• Gene Summary: This gene encodes a double-stranded-RNA-specific adenosine deaminase that is involved in editing pre-mRNAs by site-specific conversion of adenosine (A) to inosine (I). Substrates for this enzyme include ionotropic glutamate receptors (GluR2-6) and serotonin receptor (5HT2C). Studies in rodents have shown that this protein can modify its own pre-mRNA by A->I editing to create a novel acceptor splice site, alternative splicing to which results in down regulation of its protein expression. Additional splicing events result in transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (2) uses an alternate, in-frame donor splice site at one of the internal coding exons, compared to transcript variant 1. This results in a longer isoform (2) containing a 10 aa segment that is missing in isoform 1.