Add1 (NM_001024458) Mouse Untagged Clone

CAT#: MC221148

Add1 (untagged) - Mouse adducin 1 (alpha) (Add1), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Add1
• Synonyms: AI256389
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC221148 representing NM_001024458
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAATGGTGACACTCGGGCCGCAGTGGTGACCTCACCACCTCCAACCACAGCCCCTCACAAGGAGAGGT
  ATTTTGACAGAGTCGATGAGAACAACCCAGAATATTTGCGGGAGAGGAACATGGCACCAGACCTTCGTCA
  GGACTTCAATATGATGGAGCAGAAGAAGAGGGTGTCTATGATTCTACAGAGTCCTGCCTTCTGTGAAGAG
  CTGGAGTCTATGATACAGGAACAGTTTAAGAAGGGCAAGAACCCCACAGGCCTGCTGGCATTGCAGCAGA
  TTGCAGATTTCATGACTGCGAGTGTACCAAATGTCTACCCGGCAGCTCCTCAAGGAGGAATGGCCGCCCT
  GAACATGAGTCTTGGTATGGTCACTCCTGTGAATGACCTTAGAGGGTCGGATTCTATCGCATATGACAAA
  GGGGAGAAGTTACTGCGGTGTAAACTGGCAGCATTTTACAGACTGGCAGATCTCTTTGGCTGGTCCCAGC
  TTATCTACAATCATATCACAACCAGAGTGAACTCTGAGCAGGAACACTTCCTCATCGTACCTTTTGGACT
  TCTCTACAGTGAAGTGACTGCATCCAGCTTGGTTAAGGTCAATCTACAAGGAGATATAGTAGACCGTGGA
  AGTACTAACCTAGGGGTGAATCAGGCGGGCTTCACTCTGCACTCGGCAGTGTATGCTGCGCGACCAGATG
  CCAAGTGTATTGTGCACATCCACACCCCAGCGGGGGCAGCGGTCTCTGCGATGAAGTGTGGACTCTTGCC
  TATCTCCCCGGAGGCACTTTCCCTTGGAGATGTTGCTTATCATGACTATCATGGCATTCTGGTTGATGAA
  GAGGAAAAAATTTTGATTCAGAAAAATCTGGGCCCTAAAAGCAAGGTCCTCATTCTGCGGAATCACGGAC
  TCGTGTCAGTTGGAGAGAGCGTGGAGGAGGCCTTCTATTACATCCATAACCTTGTGGTTGCATGTGAGAT
  CCAGGTTCGGACTCTGGCAAGTGCTGGAGGACCAGACAACTTAGTTCTGCTGGATCCTGGGAAGTACAAA
  GCCAAATCCCGTTCTCCAGGGACTCCAGCAGGGGAAGGCTCTGGATCACCTCCCAAGTGGCAGATTGGGG
  AGCAGGAATTTGAAGCTCTTATGCGGATGCTCGATAATCTGGGCTACAGAACTGGCTACCCTTATCGATA
  CCCTGCTCTGAGAGAGAGATCTAAAAAGTACAGCGATGTGGAAGTCCCTGCCAGTGTCACAGGCCACTCC
  TTTGCTAGTGATGGCGATTCGGGCACTTGCTCGCCGCTCAGGCACAGTTTTCAGAAGCAGCAGCGAGAGA
  AGACAAGATGGCTGCACTCTGGCCGGGGTGATGACGCTTCTGAGGAGGGGCAGAACGGAAGCAGTCCCAA
  GTCGAAGACTAAGTGGACTAAAGAGGATGGACATAGAACTTCCACCTCTGCTGTCCCTAACCTGTTTGTT
  CCATTGAACACTAACCCAAAAGAGGTCCAGGAGATGAGGAACAAGATTCGAGAGCAGAACTTACAGGACA
  TTAAGACGGCAGGACCTCAGTCCCAGGTTTTGTGTGGTGTGATGATGGACAGAAGCCTTGTTCAGGGGGA
  GCTGGTGACGGCCTCCAAAGCCATCATTGAGAAGGAATACCAGCCCCATGTAATTGTGAGCACCACAGGT
  CCCAACCCCTTCAACACGCTGACAGACCGTGAACTGGAAGAATACCGACGAGAGGTGGAGCGGAAGCAGA
  AGGGCTCTGAAGAGAACCTGGATGAGACAAGAGAGCAGAAAGAGAAGAGTCCTCCAGACCAGTCAGCTGT
  CCCCAACACTCCTCCCAGTACTCCTGTCAAGCTTGAAGAAGACCTTCCCCAGGAGCCGACATCCAGGGAC
  GACAGTGATGCCACCACCTTCAAGCCGACGCCCCCTGACCTCTCCCCTGATGAGCCTTCAGAAGCACTTG
  CCTTCCCAGCAGTGGAGGAGGAAGCCCATGCTTCCCCAGACCCCACTCAGCCCCCTGCTGAGGCTGACCC
  TGAGCCAGCCTCAGCCCCAACTCCAGGAGCTGAAGAGGTAGCCTCCCCTGCCACGGAGGAGGGGTCTCCC
  ATGGACCCTGGCAGTGATGGGTCTCCAGGCAAGTCCCCATCCAAAAAGAAGAAGAAGTTCCGTACCCCGT
  CCTTCCTGAAGAAGAGCAAGAAGAAGAGTGACTCCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001024458
• Insert Size: 2208 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001024458.3, NP_001019629.2
• RefSeq Size: 3917 bp
• RefSeq ORF: 2208 bp
• Locus ID: 11518
• Cytogenetics: 5 17.9 cM
• Gene Summary: Membrane-cytoskeleton-associated protein that promotes the assembly of the spectrin-actin network. Binds to calmodulin. [UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) encodes isoform 1.