Ephb2 (NM_010142) Mouse Untagged Clone

CAT#: MC222940

Ephb2 (untagged) - Mouse Eph receptor B2 (Ephb2), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ephb2
• Synonyms: Cek5; Drt; Erk; ETECK; Hek5; Nuk; Prkm5; Qek5; Sek3; Tyro5
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC222940 representing NM_010142
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCGTGCGCAGGCTGGGGGCCGCGCTGCTGCTGCTGCCGCTGCTAGCCGCCGTGGAAGAAACCCTGA
  TGGACTCTACGACAGCAACGGCTGAGCTGGGCTGGATGGTACATCCCCCATCAGGGTGGGAAGAGGTGAG
  CGGCTACGACGAGAACATGAACACTATCCGTACCTACCAGGTGTGCAATGTCTTTGAGTCAAGCCAGAAC
  AACTGGCTGCGGACCAAATTCATCCGGCGCCGCGGCGCCCACCGCATCCACGTGGAGATGAAGTTCTCGG
  TGCGTGACTGCAGCAGCATTCCCAGCGTGCCGGGCTCCTGCAAGGAGACCTTCAACCTCTACTACTATGA
  GGCTGATTTTGACTTAGCCACCAAAACCTTTCCCAACTGGATGGAGAATCCGTGGGTGAAGGTGGACACC
  ATCGCGGCCGATGAGAGCTTCTCTCAGGTGGACCTGGGTGGCCGCGTCATGAAAATCAACACTGAGGTGC
  GAAGCTTCGGTCCCGTGTCCCGCAATGGTTTCTACCTGGCCTTCCAGGACTATGGCGGCTGTATGTCCCT
  CATTGCTGTGCGCGTCTTCTACCGGAAGTGCCCCCGAATCATCCAGAATGGTGCCATCTTCCAGGAGACA
  CTGTCGGGGGCTGAGAGCACTTCGCTGGTGGCAGCTCGGGGCAGCTGCATCGCCAATGCTGAAGAAGTGG
  ATGTGCCCATCAAACTCTACTGTAACGGGGACGGCGAATGGCTGGTGCCCATAGGTCGCTGCATGTGCAA
  GGCGGGCTTCGAGGCTGTGGAGAACGGCACCGTCTGCCGAGGTTGTCCATCAGGAACCTTCAAGGCCAAC
  CAAGGGGACGAAGCCTGCACCCACTGTCCCATCAACAGCCGCACCACCTCCGAGGGTGCCACCAACTGTG
  TATGCCGCAACGGCTACTACAGGGCCGACCTGGACCCCTTAGACATGCCTTGCACAACCATCCCCTCTGC
  GCCCCAGGCTGTGATCTCCAGCGTCAACGAGACGTCCCTCATGCTAGAGTGGACCCCACCCCGAGACTCG
  GGGGGTCGCGAGGATCTTGTTTACAACATCATCTGCAAGAGCTGTGGCTCCGGCCGGGGCGCATGCACGC
  GCTGCGGGGACAACGTGCAGTACGCGCCCCGCCAGCTGGGCCTGACTGAGCCGCGCATCTACATCAGTGA
  CCTGCTGGCACACACGCAGTACACCTTCGAGATCCAGGCCGTGAACGGTGTGACTGACCAGAGTCCCTTC
  TCACCTCAGTTCGCCTCTGTGAACATCACCACCAACCAAGCAGCACCATCGGCCGTGTCCATCATGCACC
  AGGTGAGCCGCACTGTGGACAGCATCACCCTGTCGTGGTCCCAGCCAGACCAGCCCAACGGTGTGATCCT
  GGACTACGAGCTGCAGTACTATGAGAAGGAGCTCAGTGAGTACAACGCCACGGCCATAAAAAGCCCCACC
  AACACAGTCACTGTGCAGGGCCTCAAAGCCGGCGCCATCTATGTCTTCCAGGTGCGGGCACGCACCGTTG
  CAGGCTATGGGCGCTACAGTGGCAAGATGTACTTCCAAACCATGACAGAAGCCGAGTACCAGACCAGCAT
  CAAGGAAAAGCTACCCCTCATCGTTGGCTCCTCCGCCGCCGGCTTAGTCTTCCTCATCGCTGTGGTCGTC
  ATTGCCATCGTATGTAACAGACGGGGGTTTGAGCGTGCCGACTCAGAGTACACGGACAAGCTACAACACT
  ACACCAGCGGACACATGACCCCAGGCATGAAGATCTATATAGACCCTTTCACCTATGAAGATCCTAATGA
  GGCAGTGCGGGAGTTTGCCAAGGAAATTGACATCTCCTGTGTCAAGATTGAGCAGGTGATCGGAGCAGGG
  GAATTTGGTGAGGTCTGCAGTGGCCATTTGAAGCTGCCAGGCAAGAGAGAGATCTTTGTAGCCATCAAGA
  CCCTCAAGTCAGGATACACGGAGAAACAGCGCCGGGACTTCCTGAGTGAGGCATCCATCATGGGCCAGTT
  CGACCACCCCAATGTCATCCATCTGGAAGGGGTTGTCACCAAGAGCACACCTGTCATGATCATCACTGAA
  TTCATGGAGAACGGATCTCTGGACTCCTTCCTCCGGCAAAACGATGGGCAGTTCACAGTCATCCAACTGG
  TGGGCATGCTGAGGGGCATTGCAGCCGGCATGAAGTACCTGGCGGACATGAACTACGTGCACCGTGACCT
  TGCTGCTCGAAACATCCTCGTCAACAGCAACCTGGTGTGTAAGGTGTCTGATTTTGGGCTCTCACGCTTC
  CTGGAGGATGACACGTCTGACCCCACCTATACCAGCGCTCTGGGTGGGAAGATCCCCATCCGTTGGACGG
  CACCGGAAGCCATCCAGTACCGGAAATTCACCTCGGCCAGTGATGTGTGGAGCTATGGCATCGTCATGTG
  GGAGGTGATGTCCTACGGGGAACGACCCTACTGGGACATGACCAATCAAGACGTAATCAACGCCATTGAA
  CAGGACTACAGACTACCTCCGCCCATGGACTGCCCTAGTGCCCTGCACCAGCTCATGCTGGACTGCTGGC
  AGAAGGACCGCAACCACCGGCCCAAGTTCGGCCAGATTGTCAACACGCTGGACAAGATGATCCGAAACCC
  CAACAGCCTCAAAGCCATGGCACCCCTGTCCTCTGGCATCAACCTGCCACTGCTGGACCGCACGATACCG
  GACTACACCAGCTTTAACACGGTGGATGAGTGGCTAGAGGCCATCAAGATGGGCCAGTACAAGGAGAGCT
  TTGCCAACGCCGGCTTTACCTCTTTCGACGTTGTATCTCAGATGATGATGGAGGACATTCTCCGCGTTGG
  GGTCACTCTAGCTGGCCACCAGAAAAAAATCCTGAACAGTATCCAGGTGATGCGGGCCCAGATGAACCAG
  ATCCAGTCTGTAGAGGTTTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_010142
• ORF Size: 2961 bp
• Insert Size: 2961
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info

Reference Data

• RefSeq: NM_010142.4, NP_034272.1
• RefSeq Size: 4850
• RefSeq ORF: 2961
• Locus ID: 13844
• Gene Summary: This gene encodes a member of the Eph receptor family of receptor tyrosine kinase transmembrane glycoproteins. These receptors consist of an N-terminal glycosylated ligand-binding domain, a transmembrane region and an intracellular kinase domain. The encoded receptor preferentially binds membrane-bound ephrin-B ligands and is involved in nervous system and vascular development. This gene is used as a marker of intestinal stem cells. Homozygous knockout mice for this gene exhibit impaired axon guidance and vestibular function. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2015]
  Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 5' coding region, compared to variant 1. It encodes isoform 2, which is shorter by an amino acid, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.