Adgrf3 (NM_001014394) Mouse Untagged Clone

CAT#: MC223022

Gpr113 (untagged) - Mouse G protein-coupled receptor 113 (Gpr113), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Adgrf3
• Synonyms: Gm1041; Gpr113; Pgr23
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC223022 representing NM_001014394
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCTCTCTAGCTCTCTCCCAGCTGCTCCTGGCCGTGACTCTTCCCCTGCTGGAGTTGGAGCCAACAT
  TTGTCCCCACAGCCCAATCAGAGCTCAGTCCATATGGAGGGAAATCTGGGCAGCAACTGAACCAGTACAG
  TGGAGAAGGGGAATCAGTGCTGGTCTCCAGCTATGTGCATCTGGAATTTTCAAGCACAGCCTGGCCACAG
  GAGCTCTCTAAAAACTTTACTCTTCCTACCGCCCTGGCAGTCCTTCCCCCGAAAACGCTTACTGGCCTTG
  GCCTTACAACAGAGTGTCGTGCTAACCACAGCGGAACCAGCCTCTGCACTTGCCACCCTGGCTACCAATG
  GAATGCCACACTTTGTTCCCTTTACCCACACTGCTGGGGCCGCAGGTCAGAAAGAGATTCCTGTATGTGT
  CGCAGCTTCCACGGTCCTGTTACTGGCTACTGCCAGTTGCTGCCACCTGTTCCTGCAAACCTGATACTGG
  ACTCCCAGCTGCAAATGCACGGCAACACCCTGAACCTCATTCTCCTCAAGAAAGAGGAAGCCACAAACCT
  GAGATGGTTCTTGAGACACTCAAAGAACCACACACCCATCAGCCTATGGCCAGGGACCAATGTGTTGCAG
  ACGTCCAGTGAGGGCCAGTCTGGCCTTCGTGTCGCCCGCATGTCCCGTCATTGGGCAGGTGAGTACGAGA
  GCATCTTCGAAGCCCAGGGGTTCAGGTGGAGGCTGCGGCAGCTGGTGAAGGTGCCCTTGCAGGAGGAAGA
  AGTCGTTCGACTCCCAGACGCCCTGTCCATCTCCTGTACTGCCTCCACTGGCTTTCAGCTGAGCTGCTGC
  ATCCCACTTACAACAGACTACACTGCTACCTGGAGTCCTGGGGAGGACAGCCAAGCCTCCTTGCAAAAAA
  TGTCGGATTCCCAATGCTTTGTGCTAGCTGTTCCGCACTGCCCAGCAACCAATACCACATACACCTGTAC
  CCTTCAGAGTCAGAACCTGCCTCCTCTCGTGGCTCCTGTCTCTATCACGATTATCCAGGATGGAGACACT
  ACCTGCCCTGAGGACTTCTCAGTGGTTAGCTGGAATGTCACCAAGGCTGGCTTCGTGGCACAGGCCCCAT
  GTCCTGTGAATAAGAAAGGTGTGGTAAAGAGGCTCTGTGGGACTGATGGCATCTGGAGGCCAGTCCAGAA
  CACCTGCACAGAAGCAAAGATCCTAACCTTGTGTCTTAAAGCCAAGCTGCTGGAAGGCCAGGGAAAGCCC
  TACGAGGAAGTGCCATGGATCTTGAGTGAGCTGCAAGAGCAGGTGGGCGTGGCCAGCACCCCCTCTGACT
  TATGGGAAATGCTGCACACTGTGACCTTACTGGCCAAGGTGGTAGCAGAGACAAGCACAGAGCTCACAGG
  CAGTGCTCTGAAGGACCTCCTGACGACCACAGACAAGATTCTAGACGCGAACATCAGCGCTCTGTGGACT
  CTGGCCCAGGCCCAGGAGCCCTCCATGGGCTCAGATTTCCTGAAGGCTGTGGAGACCTTGGTTCACAGCT
  TAAGACCACAACAGCATCCTTTTTCCTTCAGCTCAGCTAATGTGCTACTGCAGAGCCAGCTCCTCAGACA
  CTCCTCTCCTCCTGGCTACCAAATGTCCTTCTCCACCTGGCCCCTGCTGCAGGCACGGATTCCATGGCAT
  TCACTGGCCCCACTGGTCCATAGTGGAACTAACGTCAGTATTACTAGCCTAGTACTGCAAAAGCTGGACT
  ACCGGCTGCCCTCAAACTACACACAAGGGCTGTGGAACACCCCATACACCACCCCTGGGCTGATCCTTGT
  GGTCTCCATAACAGCAGACGGCCAGGCCTTCACGCAGGCAGAAGTTATCATGGACTATGAAGACATGAAT
  GGAACCCTTCACTGTGTGTTCTGGGACCACAGGGTCTTCCAGGGTCAGGGAGGTTGGTCAGATGAAGGGT
  GTGAGGTACATGCAGCCAATGCCAGCATCACTCAGTGCATTTGCCAGCATCTCACAGCCTTTTCCATCCT
  CATGTCCCAACACACTGTCCCAGAAAATCCCACCCTGGACCTGCTGAGCCAGGTGGGTACGGGGGCTTCC
  GTCCTGGCTTTGCTCGTCTGCCTGGCTATTTACGGGCTGGTGTGGAGAGTGGTGGTCCGGAACAAAGTCG
  CCTTCTTCCGCCATACCACCCTGTTTAACATGGTGATCTGCTTGCTGGTCGCAGACACATGCTTCTTGGG
  AAGCCCCTTCCTTCCTTCAGGCTACCACAGCCTGATCTGCCTAGTCACTGCTTTCCTGTGTCACTTTTTC
  TACCTGGCCACCTTTTTCTGGATGCTGGCACAGGCCCTGGTGCTGGCCCACCAGCTGCTTTTTGTCTTCC
  ACCAGCTGTCCAAGCACGTAGTCCTCTCACTGATGGTGATGCTGGGCTACCTGTGCCCACTGGGGTTTGC
  GGGTGTCACCCTGGGCCTCTACTTGCCTCAAAGGAAATACCTGTGGGAGGGGAAATGCTTTCTGAACGGA
  GGTGGCGTCATGCTGTACAGCTTCAGTGAGCCAGTGCTGGCCATCGTCGGTGTCAACGGGCTGGTCCTTG
  TCATCGCTGTGCTGAAGCTGCTGCGACCTTCACTGTCAGAGGGGCCCACAGTGGAGAAGCGCCAAGCTCT
  TGTGGGGGTGCTTAAAGCCCTGCTTATTCTCACACCCATCTTTGGCCTCACTTGGGGACTGGGCGTGGCC
  ACTCTGTTTGATGGCTCCATAGTCTCTCACTACGCCTTTTCCATTCTCAACAGCCTCCAGGGTGTCTTCA
  TATTAGTGTTTGGCTGCCTCACAGACAAGAAGGTACTAGAGGCCTTGCGCAAACGATTGCGAGGCAGCCG
  GTCCTCTAACTCTGCCATCTCCATGGTCACAAATGAAACCTATACCTCAGAACACAGCAAAGAAAGAAGT
  GAGCCTGCCAGTTATGAAGAAAGGATGACTGATTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001014394
• Insert Size: 2976 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001014394.2, NP_001014416.2
• RefSeq Size: 3108 bp
• RefSeq ORF: 2976 bp
• Locus ID: 381628
• Cytogenetics: 5 B1
• Gene Summary: Orphan receptor. [UniProtKB/Swiss-Prot Function]