Spred1 (NM_001277256) Mouse Untagged Clone

CAT#: MC226428

Spred1 (untagged) - Mouse sprouty protein with EVH-1 domain 1, related sequence (Spred1), transcript variant 2

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Spred1
• Synonyms: 5730461F13Rik; AL024345; AW047647
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC226428 representing NM_001277256
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCGAGGAGACGGCGACTTCTGACAACGATAATAGTTATGCACGAGTGCGAGCTGTGGTGATGACCA
  GAGATGACTCAAGTGGTGGATGGCTACCACTCGGAGGGAGCGGACTGAGCAGCGTCACTGTCTTCAGAGT
  CCCTCATCAGGAAGAGAATGGCTGTGCGGACTTTTTTATCCGTGGAGAGAGACTCAGAGACAAAATGGTG
  GTTTTGGAATGTATGCTTAAGAAAGACCTCATCTATAATAAGGTCACTCCCACATTTCACCACTGGAAGA
  TCGATGACAAGAAGTTTGGCCTTACCTTTCAGAGTCCTGCTGATGCCAGGGCTTTTGATCGAGGCATTCG
  AAGAGCTATAGAGGATATATCTCTAGGGTGCCCAGCGTCAAAAACTGAGGCTGAAGGAGGAGATGATGAT
  TTACAAACGACTGAAGAAGACACTTCCCGTTCCCTAGTGAAAGATCACTTTTTCCAGCAAGAGACAGTTG
  TTACCAGTGAACCTTACAGAAGCTCAGACATAAGACCGTTACCCTTTGAAGATCTGAATGCCAGAAGAGT
  CTACTTGCAAAGCCAAGTCAGCCAGGTGAGAAGACGAATACTGTTTCCATGCATAGCTTATATAGAAAAG
  CTGGGTGTGCTACAGAACACCAAGGCCCTCCGCTCTTTTCCATGTTACGAAACGATATAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001277256
• ORF Size: 690 bp
• Insert Size: 690
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This clone expresses the complete ORF with c-terminal tags of Myc-DDK.

Reference Data

• RefSeq: NM_001277256.1, NP_001264185.1
• RefSeq Size: 2007
• RefSeq ORF: 690
• Locus ID: 114715
• Gene Summary: This gene encodes a membrane-associated protein that is phosphorylated by tyrosine kinases in response to growth factors. The encoded protein acts as a negative regulator of the mitogen-activated protein (MAP) kinase signaling pathway. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2013]
  Transcript Variant: This variant (2) lacks two 3' coding exons and its transcription extends past a splice site that is used in variant 1, resulting in a distinct 3' coding region and 3' UTR, compared to variant 1. The encoded isoform (2) is shorter and has a distinct C-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.