Cast (NM_001301158) Mouse Untagged Clone

CAT#: MC227739

Cast (untagged) - Mouse calpastatin (Cast), transcript variant 6

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Cast
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC227739 representing NM_001301158
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCCAGTTTCTGTCTTCTACTTTCTGGGAGGGCTCACCTGCTGCAGTGTGGCAAGAAAAGCTTCGTG
  AGGGTGAACGAAAGGGAGCTGGAGAAACCATCCCTATCCTCCAGGACCATGTAATATGCTCAGAAGAAAG
  GGAACATGGGTCCAAACACCATGAGGCAAAAGCAAAAGAAGAAAGGCAGGAGAAGTGTGGTGAAGATGAG
  GACACAGTCCCAGCTGAGTACAGGTTAAAACCAGCAAAGGATAAAGATGGAAAACCACTATTGCCAGAGC
  CTGAAGAAACATCTAAGAGCCTGAGTGAGTCGGAGCTGATTGGGGAGCTTTCAGCAGATTTTGACCGATC
  TACATATCAAGACAAACCATCTACGCCAGCTGAAAAGAAATCCAATGACACATCCCAAACTCCTCCGGGG
  GAGACTGTGCCTCGGGCCTCCATGTGCAGTATACGGTCAGCGCCACCCAAACTAGCATCCTTGAAGGGCG
  TGGTACCAGAAGATGCTGTTGAAACCTTGGCTGGAAGCCTGGGGACAAGGGAAGCAGATCCAGAACATGA
  AAAAACTGTGGAGGATAAAGTCAAGGAGAAAGCTAAAGAAGAAGAGCATGAAAAACTTGGTGAAAAAGAA
  GAAACAGTGCCTCCTGATTATCGACTAGAAGAAGTCAAGGATAAGGATGGAAAACCACTCCTGCCCAAAG
  AATCCCAGGAACAACTTGCACCCTTAAGCGATGACTTCCTTCTTGATGCCTTGTCTCAGGACTTCTCCAG
  TCCGGCAAACATATCGTCTCTTGAATTTGAAGATGCCAAACTTTCTGCTGCCATTTCTGAAGTAGTTTCT
  CAGACACCTGCTCCAAGCACCCATGCAGCAGCTCCACTGCCTGGCACTGAGAAAGACAAAGAACTTGATG
  ATGCCTTGGATGAACTTTCTGACAGTCTTGGACAAAGGCCGCCTGATCCAGATGAGAACAAACCACTGGA
  TGACAAAGTGAAGGAGAAAATCAAACCAGAGCATAGCGAGAAACTGGGAGAAAGAGACGACACCATCCCC
  CCTGAATACAGGCATCTCTTGGATAATGATGGGAAGGACAAACCAGAGAAGCCACCGACTAAGAAAACAG
  AGAAACCTGATCAGGACCGGGACCCCATTGATGCCCTCTCAGAAGATTTGGATAGCTGCCCCTCAACTAC
  AGAGACCTCAAAGAATACAGCAAAGGGGAAGAGCAAGAAGACTTCAAGTTCCAAAGCATCCAAGGACGGA
  GAGAAAACAAAGGACTCTTCCAAGAAGACAGAGGAAGTGTCCAAGCCAAAGGCTAAAGAAGATGCAAGAC
  ACAGTTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001301158
• ORF Size: 1338 bp
• Insert Size: 1338
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This clone expresses the complete ORF with c-terminal tags of Myc-DDK.

Reference Data

• RefSeq: NM_001301158.1, NP_001288087.1
• RefSeq Size: 2970
• RefSeq ORF: 1338
• Locus ID: 12380
• Gene Summary: This gene encodes an inhibitor of the calcium-dependent cysteine protease, calpain. This protein plays roles in multiple processes, including apoptosis, cell cycle regulation, and membrane fusion. Multiple protein isoforms exist which contain unique N-terminal domains, and multiple inhibitory domains that share homology with each other. Some isoforms may be tissue-specific. Two different pseudogenes of this gene are found on chromosome 19. [provided by RefSeq, Jul 2014]
  Transcript Variant: This variant (6) represents use of an alternate promoter and therefore differs in the 5' UTR and 5' coding region, compared to variant 1. These differences cause translation initiation at an alternate start codon and result in an isoform (6) with a shorter and distinct N-terminus. This variant (6) also uses an alternate in-frame splice site in the coding region, compared to variant 1. The resulting protein (isoform 6) has a distinct N-terminus and is shorter than isoform 1.