Add2 (NM_001271857) Mouse Untagged Clone

CAT#: MC228779

Add2 (untagged) - Mouse adducin 2 (beta) (Add2), transcript variant 1

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Add2
• Synonyms: 2900072M03Rik; add97
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC228779 representing NM_001271857
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCGAGGACACGGTTCCCGAGGCGGCCTCCCCGCCACCCTCTCAGGGGCAGCACTACTTTGACCGGT
  TCTCTGAAGATGATCCTGAGTACTTGCGACTTCGCAACCGTGCAGCTGACCTGCGACAGGACTTCAACTT
  GATGGAGCAGAAGAAGAGGGTCACCATGATCCTGCAGAGCCCTTCTTTCAGGGAGGAGCTGGAAGGCCTC
  ATCCAGGAGCAAATGAAGAAGGGCAACAACTCCTCCAACATCTGGGCCCTCCGACAGATCGCGGACTTCA
  TGGCCAGCACCTCCCACGCAGTCTTCCCAGCTTCTTCCATGAACTTCTCCATGATGACACCCATCAACGA
  CCTCCACACTGCCGACTCCCTGAACCTGGCCAAGGGGGAGAGGCTTATGCGGTGCAAGATCAGCAGCGTC
  TACCGTCTCCTGGACCTCTACGGCTGGGCGCAGCTCAGTGACACCTACGTCACGCTGAGAGTGAGCAAGG
  AGCAGGACCACTTCCTGATCAGCCCCAAGGGGGTTTCCTGCAGCGAGGTCACAGCCTCCAGCCTGATTAA
  AGTGAACATTCTGGGAGAGGTGGTGGAGAAGGGCAGCAGTTGCTTCCCGGTGGACACCACGGGCTTCAGT
  CTGCACTCAGCCATCTATGCCGCCAGGCCCGACGTGCGGTGTGCCATCCACCTGCACACGCCTGCCACCG
  CAGCGGTGTCAGCTATGAAGTGCGGCCTCCTGCCGGTCTCCCATAATGCCCTGCTGGTGGGGGACATGGC
  CTACTATGACTTCAATGGGGAAATGGAGCAGGAAGCTGACCGAATCAACTTGCAGAAGTGCCTTGGACCC
  ACCTGCAAGATTCTGGTGCTAAGAAACCATGGCATGGTCGCCCTGGGTGACACCGTGGAGGAAGCTTTCT
  ACAAGGTCTTCCATCTGCAGGCTGCGTGTGAGGTACAGGTGTCGGCTCTGTCCAGTGCTGGGGGCACTGA
  GAACCTCATCCTCTTGGAGCAAGAGAAACACCGGCCGCACGAGGTGGGCTCTGTGCAGTGGGCCGGCAGC
  ACCTTCGGGCCCATGCAGAAGAGCCGGCTGGGAGAGCATGAATTTGAAGCCCTCATGAGGATGCTGGACA
  ATTTAGGCTACAGAACAGGCTATACGTACCGCCACCCCTTTGTCCAAGAGAAAACCAAACACAAAAGTGA
  AGTGGAGATCCCAGCCACAGTCACTGCCTTTGTGTTTGAAGAGGATGGAGTCCCAGTCCCCGCCCTGCGC
  CAGCACGCCCAGAAGCAGCAGAAGGAAAAGACCCGCTGGCTTAACACTCCCAACACCTACCTGCGGGTGA
  ACGTGGCTGACGAGGTGCAGAGGAACATGGGCAGTCCCCGACCAAAGACCACGTGGATGAAGGCTGATGA
  AGTGGAAAAGTCCAGCAGCGGCATGCCCATACGGATTGAAAACCCAAACCAATTTGTGCCTCTCTACACT
  GACCCCCAGGAAGTTCTGGACATGAGGAACAAGATTCGAGAGCAAAACCGACAAGACATAAAGTCAGCCG
  GGCCTCAGTCTCAGCTCTTGGCCAGTGTCATCGCAGAGAAGAGCCGGAGTCCGTCTACAGAGAGCCAGCT
  GATGTCCAAGGGCGATGCAGATACCAAAGATGAATCGGAGGAGACGGTGCCCAACCCCTTCAGCCAACTC
  ACTGACCAGGAGCTGGAGGAGTACAAGAAGGAGGTGGAAAGGAAGAAGCTAGAACAGGAGCAGGAAGGGG
  AGAAGGACATAGCCACAGAGAAGCCTGGTTCACCTGTAAAGTCCACACCTGCATCCCCAGTGCAGAGCCC
  ATCAAAAGCCGGGACCAAGAGCCCAGCGGTCTCTCCTTCCAAGACTTCAGAGGATACCAAGAAGACAGAA
  GTCAGCGAAGCCAACACAGAGCCTGAGCCAGTGAAACCAGAAGGGCTGGTGGTGAATGGGAAGGAGGAGG
  AGCCGAGCGTAGAAGAGGCCCTCAGCAAAGGACTGGGCCAGATGACCACCAACGCTGACACTGATGGCGA
  CAGCTACAAGGACAAAACCGAGTCAGTTACCAGTGGCCCCTTGTCCCCAGAGGGCTCGCCCTCTAAGTCA
  CCCTCCAAAAAGAAAAAGAAATTCCGAACCCCCTCGTTCCTGAAGAAAAGCAAGAAGAAGGAGAAGGTGG
  AATCCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001271857
• ORF Size: 2178 bp
• Insert Size: 2178
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This clone expresses the complete ORF with c-terminal tags of Myc-DDK.

Reference Data

• RefSeq: NM_001271857.1, NP_001258786.1
• RefSeq Size: 8223
• RefSeq ORF: 2178
• Locus ID: 11519
• Gene Summary: This gene encodes the beta subunit of the adducin family. Adducins, encoded by alpha, beta and gamma genes, are heteromeric proteins that crosslink actin filaments with spectrin at the cytoskeletal membrane. This protein, primarily found in the brain and hematopoietic cells, is regulated by phosphorylation and calmodulin interactions as it promotes spectrin assembly onto actin filaments, bundles actin and caps barbed ends of actin filaments. In mouse, deficiency of this gene can lead to mild hemolytic anemia and impaired synaptic plasticity. Mutations of this gene in mouse serve as a pathophysiological model for hereditary spherocytosis and hereditary elliptocytosis. Alternative splicing results in multiple transcript variants that encode different protein isoforms. [provided by RefSeq, Dec 2012]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (1). Variants 1, 2, 3 and 4 encode the same isoform (1, also known as beta-1).