Cast (NM_001301153) Mouse Untagged Clone

CAT#: MC228935

Cast (untagged) - Mouse calpastatin (Cast), transcript variant 1

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Cast
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC228935 representing NM_001301153
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCCAGCCCGGCCCGAAGCCTGCCGCCTCTCCCAGGCCCAGTCGCGGCGCGGCAGCTCGCCACACCC
  AGGAGCATGTTAATGAAAAGAATATTGGATCATCTTCTAAACCAGGAGAAAAGAAAGGATCCGATGAGAA
  AAAAGCAGCAAGTTTGGGGAGCAGTCAGCCTTCCAGACCACATGTTGGTGAAGCTGCCACAGCCACCAAG
  GTGACAGCTTCCTCTGCTGCCACCAGCAAGTCCCCCAGCATGAGTACCACAGAGACTAAGGCAATTCCAG
  TAAACAAACAGTTGGAAGGACCTGACCAGAAAAGACCCAGAGAACAGGCTGTGAAAACAGAGTCTAAGAA
  ACCACAATCAAGTGAGCAACCTGTGGTTCACGAGAAAAAGTCAAAAGGAGGGCCAAAGGAAGGGTCAGAG
  CCCAAAAATCTTCCGAAGCACACATCAAGTACAGGAAGCAAGCATGCTCATAAAGAAAAAGCACTTTCCA
  GATCAAATGAACAGATGGTGTCAGAGAAACCATCAGAATCAAAGACAAAATTTCAAGATGTTCCTTCAGC
  TGGTGGAGAGAGTGTGGCTGGAGGTGGGACTGTGGCCACTGCCTTGGACAAAGTGGTTGGTAAGAAAAAA
  GAACAGAAACCATTCACACCAGCTTCACCTGTGCAGTCCACACCGAGCAAGCCATCTGACAAGTCAGGCA
  TGGATGCTGCCTTGGATGACCTGATAGACACCTTAGGTGGACATGAAGATACTAACAGAGATGATCCACC
  ATATACTGGACCAGTAGTTTTGGACCCAATGTATTCTACCTACCTTGAGGCACTGGGTATAAAAGAAGGG
  ACTATTCCTCCAGAGTATAGGAAACTTTTGGAAAAAAATGAAGGGATCACACAACCTCTTCCAGACTCTC
  CTAAACCTATGGGGACTGACCAGGCTATAGATGCCTTGTCATCTGATTTCACCTGTAGTTCTCCAACTGG
  AAAGCAAAGTGAGAAAGAGAAGTCTACTGGGGAAATTTTCAAAGCTCAGTCTGCAGGAGTGACCAGAAGT
  TCTGTTCCCCCCAAGGAGAAGAAAAGGAAGGTGGAAGAGGAGGTCATAAATGATCAAGCACTTCAGGCTC
  TGTCAGATTCACTGGGCACCCGGCAGCCAGATCCTCCGAGCCACGTTAGCCAAGCTGAACAAGTCAAAGA
  GGCAAAAGCAAAAGAAGAAAGGCAGGAGAAGTGTGGTGAAGATGAGGACACAGTCCCAGCTGAGTACAGG
  TTAAAACCAGCAAAGGATAAAGATGGAAAACCACTATTGCCAGAGCCTGAAGAAACATCTAAGAGCCTGA
  GTGAGTCGGAGCTGATTGGGGAGCTTTCAGCAGATTTTGACCGATCTACATATCAAGACAAACCATCTAC
  GCCAGCTGAAAAGAAATCCAATGACACATCCCAAACTCCTCCGGGGGAGACTGTGCCTCGGGCCTCCATG
  TGCAGTATACGGTCAGCGCCACCCAAACTAGCATCCTTGAAGGGCGTGGTACCAGAAGATGCTGTTGAAA
  CCTTGGCTGGAAGCCTGGGGACAAGGGAAGCAGATCCAGAACATGAAAAAACTGTGGAGGATAAAGTCAA
  GGAGAAAGCTAAAGAAGAAGAGCATGAAAAACTTGGTGAAAAAGAAGAAACAGTGCCTCCTGATTATCGA
  CTAGAAGAAGTCAAGGATAAGGATGGAAAACCACTCCTGCCCAAAGAATCCCAGGAACAACTTGCACCCT
  TAAGCGATGACTTCCTTCTTGATGCCTTGTCTCAGGACTTCTCCAGTCCGGCAAACATATCGTCTCTTGA
  ATTTGAAGATGCCAAACTTTCTGCTGCCATTTCTGAAGTAGTTTCTCAGACACCTGCTCCAAGCACCCAT
  GCAGCAGCTCCACTGCCTGGCACTGAGCAGAAAGACAAAGAACTTGATGATGCCTTGGATGAACTTTCTG
  ACAGTCTTGGACAAAGGCCGCCTGATCCAGATGAGAACAAACCACTGGATGACAAAGTGAAGGAGAAAAT
  CAAACCAGAGCATAGCGAGAAACTGGGAGAAAGAGACGACACCATCCCCCCTGAATACAGGCATCTCTTG
  GATAATGATGGGAAGGACAAACCAGAGAAGCCACCGACTAAGAAAACAGAGAAACCTGATCAGGACCGGG
  ACCCCATTGATGCCCTCTCAGAAGATTTGGATAGCTGCCCCTCAACTACAGAGACCTCAAAGAATACAGC
  AAAGGGGAAGAGCAAGAAGACTTCAAGTTCCAAAGCATCCAAGGACGGAGAGAAAACAAAGGACTCTTCC
  AAGAAGACAGAGGAAGTGTCCAAGCCAAAGGCTAAAGAAGATGCAAGACACAGTTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001301153
• ORF Size: 2367 bp
• Insert Size: 2367
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This clone expresses the complete ORF with c-terminal tags of Myc-DDK.

Reference Data

• RefSeq: NM_001301153.1, NP_001288082.1
• RefSeq Size: 4165
• RefSeq ORF: 2367
• Locus ID: 12380
• Gene Summary: This gene encodes an inhibitor of the calcium-dependent cysteine protease, calpain. This protein plays roles in multiple processes, including apoptosis, cell cycle regulation, and membrane fusion. Multiple protein isoforms exist which contain unique N-terminal domains, and multiple inhibitory domains that share homology with each other. Some isoforms may be tissue-specific. Two different pseudogenes of this gene are found on chromosome 19. [provided by RefSeq, Jul 2014]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1).