Phpt1 (NM_029293) Mouse Tagged ORF Clone

CAT#: MG200633

Phpt1 (GFP-tagged) - Mouse phosphohistidine phosphatase 1 (Phpt1)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Phpt1
• Synonyms: 1700008C22Rik; PHP; Php14
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG200633 representing NM_029293
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCTGACCTCGGTCAGATTCCTGATGTAGACATTGATTCGGATGGCGTCTTCAAGTATGTGCTGA
  TTCGAGTCCACTTAGCAGAGCCTTCTGGGGATCCGGCGAAGGAGTGCAAGGAAATCGTGCGTGGCTACAA
  ATGGGCTGAGTACCACGCGGACATCTATGACAAAGTGTCAGGCGAGCTGCAAAGGAATGGCTATGACTGT
  GAGTGCCTAGGCGGCGGGCGCATCTCCCATCAGAGCCAGGACAGGAAGATACACGTGTATGGCTACTCTA
  TGGGTTACGGTCGTGCCCAGCACTCTGTTTCAACTGAGAAGATCAAAGCCAAGTATCCTGACTATGAGGT
  CACCTGGGCTGACGATGGCTAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG200633 representing NM_029293
  Red=Cloning site Green=Tags(s)
  
  MAADLGQIPDVDIDSDGVFKYVLIRVHLAEPSGDPAKECKEIVRGYKWAEYHADIYDKVSGELQRNGYDC
  ECLGGGRISHQSQDRKIHVYGYSMGYGRAQHSVSTEKIKAKYPDYEVTWADDGY
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_029293
• ORF Size: 372 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_029293.2, NP_083569.1
• RefSeq Size: 583
• RefSeq ORF: 375
• Locus ID: 75454