Polr1d (NM_181730) Mouse Tagged ORF Clone

CAT#: MG200671

Polr1d (GFP-tagged) - Mouse RNA polymerase 1-3 (Rpo1-3), transcript variant 2

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Polr1d
• Synonyms: 1110003G10Rik; AC19; RPA16; Rpo1-3
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG200671 representing NM_181730
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAGACGATCAGGAGCTGGAGAGGAAAGCCATAGAGGAATTGCTAAAGGAGGCAAAGCGTGGGAAAA
  CCAGAGCGGAGACAATGGGGCCGATGGGCTGGATGAAGTGTCCTCTTGCTGGTACAAATAAAAGATTCCT
  AATTAACACAATTAAGAACACACTGCCCTCCCATAAAGAGCAAGACCATGAACAAAAAGAGGGCAGTAAG
  GAGCCTGGCAAAAGCCAAGACCAGAAGGAAGCAAGTGGGAAGAAATACCGAAGTCACTCGTACAAGCGCA
  GCCTTCACTCGTCCCGGGGCTCCGCAGGCTGCTCTCCTCCACGGAAGCGGACCTCCCGGACCTCCGGGGA
  CAAGTGCGACCCACGGCCCAGCAGGCGA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG200671 representing NM_181730
  Red=Cloning site Green=Tags(s)
  
  MEDDQELERKAIEELLKEAKRGKTRAETMGPMGWMKCPLAGTNKRFLINTIKNTLPSHKEQDHEQKEGSK
  EPGKSQDQKEASGKKYRSHSYKRSLHSSRGSAGCSPPRKRTSRTSGDKCDPRPSRR
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_181730
• ORF Size: 378 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_181730.2, NP_859419.1
• RefSeq Size: 913
• RefSeq ORF: 381
• Locus ID: 20018
• Gene Summary: This gene encodes an RNA polymerase subunit that is a component of both the RNA polymerase I and RNA polymerase III complexes. RNA polymerase I is associated with transcription of pre-ribosomal RNAs, while RNA polymerase III is associated with transcription of small RNAs. Pseudogenes of this gene have been defined on chromosomes 4 and 6. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2013]