Tnnc1 (NM_009393) Mouse Tagged ORF Clone

CAT#: MG201266

Tnnc1 (GFP-tagged) - Mouse troponin C, cardiac/slow skeletal (Tnnc1)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Tnnc1
• Synonyms: AI874626; cTnC; cTnI; TnC; tncc
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG201266 representing NM_009393
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATGACATCTACAAAGCTGCGGTAGAACAGTTGACAGAGGAGCAGAAGAATGAGTTCAAGGCTGCCT
  TTGATATCTTTGTCCTGGGCGCGGAGGATGGCTGCATCAGCACCAAGGAGCTGGGCAAGGTGATGAGGAT
  GCTGGGCCAGAACCCCACACCTGAGGAGCTGCAGGAGATGATTGACGAAGTAGACGAGGATGGCAGTGGC
  ACAGTGGACTTCGATGAGTTTCTTGTCATGATGGTTCGGTGCATGAAGGACGACAGCAAAGGGAAGTCTG
  AGGAGGAGCTGTCGGATCTCTTCCGCATGTTTGACAAAAACGCTGATGGCTACATTGACTTAGATGAGCT
  GAAGATGATGCTGCAGGCCACAGGTGAGACCATTACGGAAGATGACATTGAAGAGCTCATGAAGGACGGT
  GACAAGAACAACGATGGCCGAATTGACTATGACGAGTTCCTGGAATTCATGAAGGGTGTGGAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG201266 representing NM_009393
  Red=Cloning site Green=Tags(s)
  
  MDDIYKAAVEQLTEEQKNEFKAAFDIFVLGAEDGCISTKELGKVMRMLGQNPTPEELQEMIDEVDEDGSG
  TVDFDEFLVMMVRCMKDDSKGKSEEELSDLFRMFDKNADGYIDLDELKMMLQATGETITEDDIEELMKDG
  DKNNDGRIDYDEFLEFMKGVE
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_009393
• ORF Size: 483 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_009393.2, NP_033419.1
• RefSeq Size: 703
• RefSeq ORF: 486
• Locus ID: 21924
• Gene Summary: Troponin is the central regulatory protein of striated muscle contraction. Tn consists of three components: Tn-I which is the inhibitor of actomyosin ATPase, Tn-T which contains the binding site for tropomyosin and Tn-C. The binding of calcium to Tn-C abolishes the inhibitory action of Tn on actin filaments. [UniProtKB/Swiss-Prot Function]