Aprt (NM_009698) Mouse Tagged ORF Clone

CAT#: MG201601

Aprt (GFP-tagged) - Mouse adenine phosphoribosyl transferase (Aprt)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Aprt
• Synonyms: C85684
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG201601 representing NM_009698
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCGGAACCTGAGTTGAAACTGGTGGCGCGGCGCATCCGCAGCTTCCCCGACTTCCCAATCCCGGGCG
  TGCTGTTCAGGGATATCTCGCCCCTCTTGAAAGACCCGGACTCCTTCCGAGCTTCCATCCGCCTCTTGGC
  CAGTCACCTGAAGTCCACGCACAGCGGCAAGATCGACTACATCGCAGGTCTAGACTCCAGGGGCTTCCTG
  TTTGGCCCTTCCCTAGCTCAGGAGCTGGGCGTGGGCTGTGTGCTCATCCGGAAACAGGGGAAGCTGCCGG
  GCCCCACTGTGTCAGCCTCCTATTCTCTGGAGTATGGGAAGGCTGAGCTGGAAATCCAGAAAGATGCCTT
  GGAACCCGGGCAGAGAGTGGTCATTGTGGATGACCTCCTGGCCACAGGAGGAACCATGTTTGCGGCCTGT
  GACCTGCTGCACCAGCTCCGGGCTGAAGTGGTGGAGTGTGTGAGCCTGGTGGAGCTGACCTCGCTGAAGG
  GCAGGGAGAGGCTAGGACCTATACCATTCTTCTCTCTCCTCCAGTATGAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG201601 representing NM_009698
  Red=Cloning site Green=Tags(s)
  
  MSEPELKLVARRIRSFPDFPIPGVLFRDISPLLKDPDSFRASIRLLASHLKSTHSGKIDYIAGLDSRGFL
  FGPSLAQELGVGCVLIRKQGKLPGPTVSASYSLEYGKAELEIQKDALEPGQRVVIVDDLLATGGTMFAAC
  DLLHQLRAEVVECVSLVELTSLKGRERLGPIPFFSLLQYD
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_009698
• ORF Size: 540 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_009698.2, NP_033828.2
• RefSeq Size: 866
• RefSeq ORF: 543
• Locus ID: 11821
• Gene Summary: Catalyzes a salvage reaction resulting in the formation of AMP, that is energically less costly than de novo synthesis. [UniProtKB/Swiss-Prot Function]