Arhgdib (NM_007486) Mouse Tagged ORF Clone

CAT#: MG202044

Arhgdib (GFP-tagged) - Mouse Rho, GDP dissociation inhibitor (GDI) beta (Arhgdib)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Arhgdib
• Synonyms: D4; Gdid4; Ly-GDI
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG202044 representing NM_007486
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACGGAGAAGGATGCACAGCCACAGCTGGAAGAGGCGGACGACGACCTGGACAGCAAGCTCAATTATA
  AGCCACCCCCTCAGAAGTCCTTGAAGGAGCTGCAGGAGATGGACAAGGATGACGAGAGTCTAACCAAGTA
  CAAGAAAACACTGCTGGGAGATGTCCCTGTGGTAGCAGACCCAACAGTTCCCAATGTGACTGTTACCCGG
  CTTAGCCTTGTATGTGACAGTGCACCAGGACCCATCACCATGGACCTTACTGGGGATCTCGAGGCCCTCA
  AAAAGGATACATTTGTGCTAAAGGAAGGCATTGAATACAGGGTGAAAATTAACTTCAAAGTGAATAAGGA
  TATTGTGTCTGGCCTGAAGTATGTTCAACACACATACCGGACTGGCATGAGAGTGGATAAAGCCACATTC
  ATGGTTGGCAGCTATGGGCCCCGACCAGAGGAGTACGAATTCCTCACTCCAGTAGAGGAAGCTCCCAAGG
  GCATGCTGGCCCGAGGCACTTACCACAACAAGTCCTTCTTCACGGATGACGACAAACAGGACCACCTCAC
  CTGGGAATGGAACCTGGCCATTAAGAAGGATTGGACAGAA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG202044 representing NM_007486
  Red=Cloning site Green=Tags(s)
  
  MTEKDAQPQLEEADDDLDSKLNYKPPPQKSLKELQEMDKDDESLTKYKKTLLGDVPVVADPTVPNVTVTR
  LSLVCDSAPGPITMDLTGDLEALKKDTFVLKEGIEYRVKINFKVNKDIVSGLKYVQHTYRTGMRVDKATF
  MVGSYGPRPEEYEFLTPVEEAPKGMLARGTYHNKSFFTDDDKQDHLTWEWNLAIKKDWTE
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_007486
• ORF Size: 600 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_007486.1, NP_031512.1
• RefSeq Size: 1099
• RefSeq ORF: 603
• Locus ID: 11857
• Gene Summary: The protein encoded by this gene is a member of the Rho guanine nucleotide dissociation inhibitor (GDI) family. This gene is expressed at high levels in hematopoietic cells. This protein is cytosolic, and dissociation of Rho from this protein is required for membrane association and activation of Rho by Guanine Nucleotide Exchange Factors (GEFs). C-terminal truncations of this gene product have been reported to promote metastasis. Multiple transcript variants and protein isoforms exist. [provided by RefSeq, Aug 2014]