Arhgdig (BC002032) Mouse Tagged ORF Clone

CAT#: MG202640

Arhgdig (GFP-tagged) - Mouse Rho GDP dissociation inhibitor (GDI) gamma (cDNA clone MGC:5923 IMAGE:3490167)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Arhgdig
• Synonyms: RIP2, Rho-GDI2, Rho-GDI-3
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG202640 representing BC002032
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGGGCCTGGACGCGTGTGAGCTGGGGGAGCAGCTGTTGGAGCTGCTCCGGCTGGCGCTGTGCGCCC
  GAGTCCTCTTGGCTGACAAGGATGGGGAGTCAACTCCATCAGATGAGGTGCTGGACGAAATTGTACCAGA
  GTACCAGGCCCCTGGGAAGAAGAGCATGCTGGCAATCTGGCAACTGGACCCCGGGGACGTGAGCTTGGTC
  AAGTACAAGCAGGCGCTGCTGGGACCCCTGCCACCCATTATGGACCCCAGCCTGCCCAACGTGCAGGTGA
  CTAGGCTGACACTGCTGACGGAGCAGGCTCCAGGGCCTATCATCATGGACCTTACAGGAGACCTAGATGC
  ACTGAAAAACCAGGTGTTTGTCCTGAAGGAGGGCATTGAATACAAGGTGAAGATCACCTTCAAGGTCAAC
  AAGGAGATTGTCAGTGGCCTCAAATGTCTGCATCACACCTATCGTCGGGGACTGCGTGTGGACAAGGCCA
  TCTTCATGGTGGGAAGCTACGGCCCCAGAGCCCAGGAGTATGAATTTGTGACTTCAGTGGAGGAAGCACC
  AAGAGGAGCATTGGCACGTGGCCTCTACGTGGTCAGGTCCCTCTTCACTGATGACGACAGGCTGAACCAC
  CTGTCCTGGGAGTGGCACCTCCATGTCTGCCAGGACTGGAAGGAC
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG202640 representing BC002032
  Red=Cloning site Green=Tags(s)
  
  MLGLDACELGEQLLELLRLALCARVLLADKDGESTPSDEVLDEIVPEYQAPGKKSMLAIWQLDPGDVSLV
  KYKQALLGPLPPIMDPSLPNVQVTRLTLLTEQAPGPIIMDLTGDLDALKNQVFVLKEGIEYKVKITFKVN
  KEIVSGLKCLHHTYRRGLRVDKAIFMVGSYGPRAQEYEFVTSVEEAPRGALARGLYVVRSLFTDDDRLNH
  LSWEWHLHVCQDWKD
  
  SGPTRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: BC002032
• ORF Size: 677 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: BC002032, AAH02032
• RefSeq Size: 1049
• RefSeq ORF: 677
• Locus ID: 14570
• Gene Summary: Inhibits GDP/GTP exchange reaction of RhoB. Interacts specifically with the GDP- and GTP-bound forms of post-translationally processed Rhob and Rhog proteins, both of which show a growth-regulated expression in mammalian cells. Stimulates the release of the GDP-bound but not the GTP-bound RhoB protein. Also inhibits the GDP/GTP exchange of RhoB but shows less ability to inhibit the dissociation of prebound GTP. [UniProtKB/Swiss-Prot Function]